Lab Papers

Rosslyn Grosely*, Carlos Alvarado*, Ivaylo P. Ivanov, Oliver B. Nicholson, Joseph D. Puglisi, Thomas E. Dever#, and Christopher P. Lapointe#. eIF1 and eIF5 dynamically control translation start site fidelity. 2024. bioRxiv preprint: https://doi.org/10.1101/2024.07.10.602410.

Chris’ First-Author Papers

Lapointe CP, Grosely R, Sokabe M, Alvarado C, Wang J, Montabana E, Villa N, Shin B-S, Dever TE, Fraser CS, Fernández IS, and Puglisi JD. 2022. eIF5B and eIF1A reorient initiator tRNA to allow ribosomal subunit joining. Nature. 607, 185-190. https://doi.org/10.1038/s41586-022-04858-z

Lapointe CP, Grosely R, Johnson AG, Wang J, Fernández IS, and Puglisi JD. 2021. Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation. Proceedings of the National Academy of Sciences, USA. 118 (6), e2017715118. doi.org/10.1073/pnas.2017715118.

• Highlighted in: Zimmer, C. The New York Times, October 9, 2020. The Coronavirus unveiled.

Johnson AG*, Lapointe CP*, Wang J, Corsepius N, Choi J, Fuchs G, and Puglisi JD. 2019. RACK1 on and off the ribosome. RNA. 25, 881-895. doi: 10.1261/rna.071217.119 *Contributed equally

• Featured article: July 2019 issue of RNA.

Lapointe CP*, Stefely JA*, Jochem A, Hutchins PD, Wilson GM, Kwiecien NW, Coon JJ, Wickens M, and Pagliarini DJ. 2018. Multi-omics reveal specific targets of the RNA-binding protein Puf3p and its orchestration of mitochondrial biogenesis. Cell Systems. 6, 125-135. * Contributed equally.

• Highlighted in: Matmiller, B. December 13, 2017. Cracking the code of coenzyme Q biosynthesis.

Lapointe CP and Wickens M. 2018. RNA Tagging: Preparation of high-throughput sequencing libraries. Methods in Molecular Biology. 1649:455-471. doi: 10.1007/978-1-4939-7213-5_30.

Lapointe CP, Preston MA, Wilinski D, Saunders HAJ, Campbell ZT, and Wickens M. 2017. Architectural principles and dynamics of a protein-RNA super-network. RNA. 23, 1636-1647. doi:10.1261/rna.062687.117.

• Featured article: November 2017 issue of RNA.

Lapointe CP, Wilinski D, Saunders HAJ, and Wickens M. 2015. Protein-RNA networks revealed through covalent RNA marks. Nature Methods. 12, 1163-1170. doi:10.1038/nmeth.3651.

Highlighted in:

• Rusk, N. 2016. Nature Methods, 13, 466-467. doi:10.1038/nmeth.3887.
• Osorio, J. 2016. Nature Reviews Genetics, 17, 6-7. doi:10.1038/nrg.2015.6.
• Williams, R. The Scientist Magazine, July 1, 2016. A New way to ID Targets of RBPs.
• Kumar, A. 2015. F1000 recommended article. http://f1000.com/prime/725901630#abstract

Lapointe CP and Wickens M. 2013. The nucleic acid-binding domain and translational repression activity of a Xenopus terminal uridylyl transferase. Journal of Biological Chemistry. Jul 12;288(28):20723-33. doi: 10.1074/jbc.M113.455451.

Chris’ Supporting-Author Papers

Schubert K*, Karousis ED*, Ban I, Lapointe CP, Leibundgut M, Bäumlin E, Scaiola A, Schönhut T, Ziegelmüller J, Puglisi JD, Mühlemann O, and Ban N. 2023. Universal features of Nsp1-mediated translational shutdown by coronaviruses. Accepted. Preprint: bioRxiv: https://www.biorxiv.org/content/10.1101/2023.05.31.543022v1

Hentschel J, Choi J, Bagshaw CR, Lapointe CP, Wang J, Jansson LI, Badstuebner M, Puglisi JD, and Stone MD. Multiplexed single-molecule analysis of human telomerase synthesizing DNA. Submitted. Preprint: bioRxiv: https://www.biorxiv.org/content/10.1101/2021.06.25.449954v1

Medina-Muñoz HC, Lapointe CP, Porter D, and Wickens M. 2020. Records of RNA localization through covalent tagging. Proceedings of the National Academy of Sciences, USA. 38, 23539-23547. doi.org/10.1073/pnas.1921408117

Johnson AG, Flynn RA, Lapointe CP, Ooi YS, Zhao ML, Richards CM, Qiao W, Yamada SB, Couthouis J, Gitler AD, Carette JE, and Puglisi JD. 2019. A memory of eS25 loss drives resistance phenotypes. Nucleic Acids Research, 48, 13:7279-7297. doi.org/10.1093/nar/gkaa444

Wang J, Johnson AG, Lapointe CP, Choi J, Prabhakar A, Chen DH, Petrov, AN, and Puglisi JD. 2019. eIF5B gates the transition from translation initiation to elongation. Nature, 573, 605-608. doi.org/10.1038/s41586-019-1561-0.

Preston MA, Porter DF, Chen F, Buter N, Lapointe CP, Keles S, Kimble J, and Wickens M. 2019. Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase. Nature Methods. 16, 437-445. doi.org/10.1038/s41592-019-0370-6

Choi J, Grosely R, Prabhakar A, Lapointe CP, Wang J, Puglisi JD. 2018. How mRNA and nascent chain sequences regulate translation elongation. Annual Review of Biochemistry. 87, 421-449. doi: 10.1146/annurev-biochem-060815-014818

Wilinksi D, Buter N, Klocko A, Lapointe CP, Selker E, Gasch AP, and Wickens M. 2017. Recurrent rewiring and emergence of RNA regulatory networks. Proceedings of the National Academy of Sciences, USA. doi:10.1073/pnas.1617777114.

Wilinski D*, Qiu C*, Lapointe CP, Nevil M, Campbell ZT, Hall T, and Wickens M. 2015. RNA regulatory networks diversified through curvature of the PUF protein scaffold. Nature Communications. 6:8213 doi: 10.1038/ncomms9213. * Contributed equally