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 Yu-Chien Chuang , Gerald R Smith 
Dynamic configurations of meiotic DNA-break hotspot determinant proteins
J Cell Sci  2022 Feb 1;135(3):jcs259061.  doi: 10.1242/jcs.259061. Epub 2022 Feb 7.  PMID: 35028663


Randy W Hyppa, Joshua D Cho, Mridula Nambiar, Gerald R Smith
Redirecting meiotic DNA break hotspot determinant proteins alters localized spatial control of DNA break formation and repair.
Nucleic Acids Research, gkab1253,

Wintrebert M, Nguyen MC, Gerald R Smith GR, Activation of meiotic recombination by nuclear import of the DNA break hotspot-determining complex in fission yeast J. Cell Sci. Feb 22;134(4):jcs253518. doi: 10.1242/jcs.253518 PMID: 33526714    


Amundsen SK, Taylor AF, Smith GR. Chi hotspot control of RecBCD helicase-nuclease by long-range intramolecular signaling. Sci Rep. 2020 Nov 5;10(1):19415. doi: 10.1038/s41598-020-73078-0. PMID: 33154402 PMCID: PMC7644769

Karabulut AC, Cirz RT, Taylor AF, Smith GR
, Small-molecule sensitization of RecBCD helicase-nuclease to a Chi hotspot-activated state. Nucleic Acids Research. 2020 48: 7973-7980  PMCID: PMC7641324

Smith GR, Nambiar M. New Solutions to Old Problems: Molecular Mechanisms of Meiotic Crossover Control. Trends in Genetics. 2020 36: 337-346 PMCID: PMC7162993.


Nambiar M, Chuang YC, Smith GR. Distributing meiotic crossovers for optimal fertility and evolution. 2019 Sep;81:102648. doi: 10.1016/j.dnarep.2019.102648. Epub 2019 Jul 8 PMID: 31345733

Amundsen SK, Smith GR. The RecB helicase-nuclease tether mediates Chi hotspot control of RecBCD enzyme. Nucleic Acids Res. 2019 Jan 10;47(1):197-209. doi: 10.1093/nar/gky1132. PMID: 30445486 PMCID:PMC6326792  Abstract | PDF


Fowler KR, Hyppa RW, Cromie GA, Smith GR. Physical basis for long-distance communication along meiotic chromosomes PNAS October 2, 2018 115 (40) E9333-E9342 PMID:30217891
| Abstract

Nambiar M, Smith GR. Pericentromere-specific cohesin complex prevents meiotic pericentric DNA double-strand breaks and lethal crossovers. Mol Cell  2018 Aug 16;71(4):540-553.e4. doi: 10.1016/j.molcel.2018.06.035. Epub 2018 Aug 2. PMID: 30078721


Nuckolls NL, Bravo Núñez MA, Eickbush MT, Young JM, Lange JJ, Yu JS, Smith GR, Jaspersen SL, Malik HS, Zanders SE. wtf genes are prolific dual poison-antidote meiotic drivers.eLife 2017. PMCID PMC5478261 | Abstract

Ma L, Fowler KR, Martin-Castellanos C, Smith GR.  Functional organization of protein determinants of meiotic DNA break hotspots. Sci Rep, (2017) 7(1), 1393. Abstract

Hyppa RW, Fowler KR, Smith GR. Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein Binding in S. pombe.Methods Mol Biol, (2017) 1471, 25-49. Abstract  


Taylor AF, Amundsen SK, Smith GR. Unexpected DNA context-dependence identifies a new determinant of Chi recombination hotspots. Nucleic Acids Research, 2016 doi: 10.1093/nar/gkw541 PMCID PMC5041463 | PDF

Polakova S, Molnarova L, Hyppa RW, Benko Z, Misova I, Schleiffer A, Smith GR, Gregan J. Dbl2 Regulates Rad51 and DNA Joint Molecule Metabolism to Ensure Proper Meiotic Chromosome Segregation PLoS Genet 2016 12(6): e1006102. PMCID: PMC4909299 | PDF

Amundsen SK, Sharp JW, Smith GR.  RecBCD Enzyme "Chi Recognition" Mutants Recognize Chi Recombination Hotpots in the Right DNA Context. Genetics. (2016) 204: 139-152 PMCID: PMC5012381 | PDF

Nambiar M, Smith GR, Repression of harmful meiotic recombination in centromeric regions. Seminars in Cell & Dev Biol. (2016) 54: 188-197 PMCID: PMC4867242 | PDF

Hyppa RW, Fowler KR, Smith GR, Quantitative genome-wide measurements of meiotic DNA double-strand breaks and protein-binding in S. pombe, Meiosis, 2nd Edition, D.T. Stuart, ed. (Springer), in press


Ma L, Milman N, Nambiar M, Smith GR. Two separable functions of Ctp1 in the early steps of meiotic DNA double-strand break repair. Nucleic Acids Res. 2015 Sep 3;43(15):7349-59. PubMed PMID: 26130711; PMCID: PMC4551917 | PDF

Phadnis N, Cipak L, Polakova S, Hyppa RW, Cipakova I, Anrather D, Karvaiova L, Mechtler K, Smith GR, Gregan J. Casein Kinase 1 and Phosphorylation of Cohesin Subunit Rec11 (SA3) Promote Meiotic Recombination through Linear Element Formation. PloS Genetics (2015) May 20;11(5):e1005225 PMCID: PMC4439085 | PDF


Zanders SE, Eickbush MT, Yu JS, Kang JW, Fowler KR, Smith GR, Malik HS.  Genome rearrangements and pervasive meiotic drive cause hybrid infertility in fission yeast. Elife. (2014) 3: e02630 PMCID: PMC4066438 | PDF

Fowler KR, Sasaki M, Milman N, Keeney S, Smith GR. Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome. Genome Research 10: 1650-64 (2014). PMCID: PMC4199369 | PDF

Taylor A, Amundsen SK, Guttman M, Lee, KK, Luo, J, Rannish J Smith, GR. Control of RecBCD Enzyme Activity by DNA Binding- and Chi Hotspot-Dependent Conformational Changes. Journal of Molecular Biology 21: 3479-99 (2014) PMCID: PMC4188757 | PDF

Cipak L, Polakova S, Hyppa RA, Smith GR, Gregan J. Synchronized fission yeast meiosis using an ATP analog-sensitive Pat1 protein kinase. Nat Protoc (2014) 9: 223-231 PMCID: PMC4117242 | PDF


Hyppa RW, Fowler KR, Cipak L, Gregan J, Smith GR  DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperatureNucleic Acids Res. (2013) 42: 359-69 PMID: 24089141 | PDF

Bannister TD, Nair R, Spicer T, Fernandez Vega V, Eberhart C, Mercer BA, Cameron M, Schurer S, Amundsen SK, Karabulut A, Londoo LM, Smith GR, Hodder P. ML328: A Novel Dual Inhibitor of Bacterial AddAB and RecBCD Helicase-nuclease DNA Repair Enzymes. Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. 2012 Dec 17 [updated 2013 Apr 5]. PMID: 23833795 Link

Martín-Castellanos C, Fowler KR, Smith GR. Making chromosomes hot for breakage. Cell Cycle. 2013 May 1;12(9):1327-8. doi: 10.4161/cc.24576. Epub 2013 Apr 11. No abstract available. PMID: 23588069 Link

Smith GR. Bruce Ames. Brenner's Online Encyclopedia of Genetics Second Edition; S. Maloy and K. Hughes, eds. Elsevier Press (2013)

Smith GR. RecBCD enzyme and pathway. Brenner's Online Encyclopedia of Genetics (Second Edition; S. Maloy and K. Hughes, eds.) Elsevier Press. (2013)

Smith GR. Chi sequences, Brenner's Online Encyclopedia of Genetics (Second Edition; S. Maloy and K. Hughes, eds. Elsevier Press) (2013) 

Fowler KR, Gutiérrez-Velasco S, Martín-Castellanos C,  Smith GR. Protein Determinants of Meiotic DNA Break Hotspot. Mol Cell (2013) 49: 983-996  PMCID: PMC3595357   Additional_Supplemental_Data


Cipak L, Hyppa RW, Smith GR, Gregan J. ATP analog-sensitive Pat1 protein kinase for synchronous fission yeast meiosis at physiological temperature. Cell Cycle 11: 1626-1633 (2012) PMCID: PMC3341230

Wehrkamp-Richter S, Hyppa RW, Prudden J, Smith GR, Boddy MN. Meiotic DNA joint molecule resolution depends on Nse5-Nse6 of the Smc5-Smc6 holocomplex. Nucleic Acids Res.  40: 9633-9646 (2012) PMCID:PMC3479181 | PDF

Smith GR. How RecBCD and Chi promote DNA break repair and recombination – A molecular biologist’s view. Microbiology and Molecular Biology Reviews 75: 217-228 (2012)  PMCID: PMC3372252

Amundsen SK, Spicer T, Karabulut AC. Londoño LM, Eberhardt C, Fernandez Vega V Bannister TD, Hodder P, Smith GR. Small-molecule inhibitors of bacterial AddAB and RecBCD helicase-nuclease DNA repair enzymes. ACS Chemical Biology 7: 879-891 (2012) PMCID: PMC3356449


Phadnis N, Hyppa RW, Smith GR. New and old ways to control meiotic recombination. Trends Genet 27: 411-421 (2011) PMCID: PMC3177014

Reddy BD, Wang Y, Niu L, Higuchi EC, Marguerat SB, Bähler J,  Smith GR, Jia S. Elimination of a specific histone H3K14 acetyltransferase complex bypasses the RNAi pathway to regulate pericentric heterochromatin functions. Genes Dev 25: 214-219 (2011) PMCID: PMC3034895

Bonfils S, Rozalén AE, Smith GR, Moreno S, Martin-Castellanos C. Functional interactions of Rec24, the fission yeast ortholog of mouse Mei4, with the meiotic recombination-initiation complex. Journal of Cell Science 124:1328-1338 (2011) PMCID: PMC3065387


Hyppa RW, Smith GR. Crossover invariance determined by partner choice for meiotic DNA break repair. Cell 142: 243-55 (2010) PMCID: PMC2911445 | 
PDF file

Ellermeier C, Higuchi EC, Phadnis N, Holm L, Geelhood JL, Thon G, Smith GR. RNAi and heterochromatin repress centromeric meiotic recombination PNAS 107: 8701-8705 (2010) PMCID: PMC2889303 | PDF file 


Milman N, Higuchi E, Smith GR. Meiotic double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size-class of Rec12 (Spo11)-oligonucleotides. Mol Cell Biol.  29: 5998-6005 (2009) PMCID: PMC2772569 PDF file 

Cromie G. Phylogenetic ubiquity and shuffling of the bacterial RecBCD and AddAB recombination complexes. J Bacteriol. 16: 5076-84. (2009) PMCID: PMC2725590 PDF file 

Hyppa RW,  Smith GR. Using Schizosaccharomyces pombe meiosis to analyze DNA recombination intermediates, in “Meiosis vol. 1, Molecular and Genetic Methods” (ed. S. Keeney) Humana press.  235-252 (2009) PMCID: PMC2758538

Smith GR. Genetic analysis of meiotic recombination in Schizosaccharomyces pombe, in “Meiosis vol. 1, Molecular and Genetic Methods” (ed. S. Keeney) Humana Press  65-76 (2009)

Amundsen SK, Fero J, Salama NR, Smith GR. Dual nuclease and helicase activities of helicobacter pylori AddAB are required for DNA repair, recombination, and mouse infectivity. J. Biol. Chem. 284: 16759–16766 (2009) PMCID: PMC2719311 PDF file

Farah JA, Cromie, GA,  Smith GR. Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination. Proc. Natl. Acad. Sci. 106: 9356-9361 (2009) PMCID: PMC2695049 PDF file


Hyppa RW, Cromie GA, Smith GR. Indistinguishable Landscapes of Meiotic DNA Breaks in rad50+ and rad50S Strains of Fission Yeast Revealed by a Novel rad50+ Recombination Intermediate. PLoS Genet 4: (2008) e1000267. Epub PMCID: PMC2580034

Smith GR. Meeting DNA palindromes head-to-head. Genes & Development 22: 2612-2620 (2008) pdf file

Cromie GA, Smith, GR. Meiotic recombination in S. pombe: A paradigm for genetic and molecular analysis, in Recombination and Meiosis. In Genome Dynamics and Stability, R. Egel, D-H Lankenau eds. (Springer-Verlag), pp. 195-230 (2008) pdf file

Cromie GA, Hyppa RW,  Smith GR. The fission yeast BLM homolog Rqh1 promotes meiotic recombination. Genetics 179: 1157-1167. (2008) PMCID: PMC2475723 pdf file

Oh SD, Lao, JP, Taylor AF, Smith GR, Hunter N. RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell 31: 324-336 (2008) PMCID: PMC2587322  

Amundsen SK, Fero J, Hansen LM, Cromie GA, Solnick JV, Smith GR, Salama NR. Helicobacter pylori AddAB helicase-nuclease and RecA promote recombination-related DNA repair and survival during stomach colonization. Molec Microb 69: 994-1007 (2008) PMCID: PMC2680919 pdf file

Davis LD, Rozalen AE, Moreno S, Smith GR, Martin-Castelanos C. Rec25 and Rec27, novel linear element components, link cohesin to meiotic DNA breakage and recombination. Current Biology 18: 849-854 (2008) pdf file


Amundsen S, Taylor AF, Reddy M, Smith GR. Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hotspots

Genes & Development 21: 3296-3307 (2007) PMCID: PMC2113030 pdf file

Cromie G, Hyppa RW, Hugh, CP, Farah JA, Grewal SI, Smith GR. A discrete class of intergenic DNA dictates meiotic DNA break hotspots in fission yeast. PLoS Genetics 3: e141 (2007) PMCID: PMC1950956  pdf file

Cromie G, and Smith GR. Branching out: meiotic recombination and its regulation. TRENDS in Cell Biology17: 448-455 (2007) pdf file

Oh SD, Lao JP, Hwang Yi-Hwa Hwang P, Taylor AF, Smith GR, Hunter N. Sgs1, a Bloom’s helicase ortholog, prevents aberrant crossing-over by suppressing the formation of multi-chromatid joint molecules. Cell 130: 259-272 (2007). PMCID: PMC2034285 pdf file

Amundsen SK,  Smith GR. Chi Hotspot activity in Escherichia coli without RecBCD exonuclease activity: Implications for the mechanism of recombination. PMCID: PMC1774988. Genetics 175: 41-54 (2007). Abstract


Cromie G, Hyppa RW, Taylor AF, Zakharyevich K, Hunter N,  Smith GR. Single Holliday junctions are intermediates of meiotic recombination. Cell 127: 1167-1178 (2006) Abstract | pdf file

Davis L, Smith, GR. The meiotic bouquet promotes homolog interactions and restricts ectopic recombination in Schizosaccharomyces pombe. Genetics 174: 167-177 (2006), PMCID: PMC1569800 Abstract  


Steiner WW, and Smith GR. Natural meiotic recombination hot spots in the Schizosaccharomyces pombe genome successfully predicted from the simple sequence motif M26Mol. Cell. Biol. 25, 9054-9062. (2005) PMCID: PMC1265782. Abstract | pdf file

Martin-Castellanos C, Blanco M, Rozalen AE, Perez-Hidalgo L, Garcia AI, Conde F, Mata J, Ellermeier C, Davis L, San-Segundo P, Smith GR, Moreno S. A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events. Current Biol22, 2056-2062 (2005) Abstract

Farah JA, Cromie G, Davis L, Steiner WW, Smith GR. Activation of an alternative, Rec12 (Spo11)-independent pathway of fission yeast meiotic recombination in the absence of a DNA flap endonuclease. Genetics 171, 1499-1511 (2005). PMCID: PMC1456079, Abstract

Ellermeier C, Smith GR. Cohesins are required for meiotic DNA breakage and recombination in Schizosaccharomyces pombe. Proc. Natl. Acad. Sci.102: 10952-10957 (2005) PMCID: PMC1182449 Abstract | pdf file

Malapeira J, Moldon A, Hidalgo E, Smith GR, Nurse P, Ayte J. A meiosis-specific cyclin regulated by splicing is required for proper progression through meiosis. Mol. Cell. Biol. 25: 6330-6337 (2005) PMCID: PMC1190344 Abstract

Davis L, Smith GR. Dynein promotes achiasmate segregation in Schizosaccharomyces pombeGenetics 170: 581-590 (2005). PMCID: PMC1450395 Abstract

Steiner WW, Smith GR. Optimizing the nucleotide sequence of a meiotic recombination hotspot in Schizosaccharomyces pombeGenetics 169: 1973-1983 (2005) PMCID: PMC1449614 Abstract | pdf file

Farah JA, Cromie G, Steiner WW, Smith GR. A novel recombination pathway initiated by the Mre11/Rad50/Nbs1 complex eliminates palindromes during meiosis in Schizosaccharomyces pombeGenetics 169: 1261-1274 (2005) PMCID: PMC1449568 Abstract | pdf file

Cromie G, Rubio, C, Hyppa RW, Smith GR. A natural meiotic DNA break site in Schizosaccharomyces pombe is a hotspot of gene conversion, highly associated with crossing-over. Genetics 169: 595-605 (2005) Abstract  | pdf file


Duquette ML, Handa P, Vincent JA, Taylor AF, Maizels N. Intracellular transcription of G-rich DNAs induces formation of G-loops, novel structures containing G4 DNA Genes and Development 18: 1618-29 (2004) Abstract

Ellermeier C, Schmidt H, Smith GR. Swi5 acts in meiotic DNA joint molecule formation in Schizosaccharomyces pombeGenetics 168: 1891-1898 (2004) PMCID: PMC1448720 Abstract

Young JA, Hyppa RW, Smith GR. Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts. Genetics 167: 593-605 (2004) PMCID: PMC1470912 Abstract

Smith GR. How homologous recombination is initiated: Unexpected evidence for single-strand nicks from V(D)J site-specific recombination. Cell 117: 146-148 (2004) Abstract


Smith GR, Boddy MN, Shanahan P, Russell P. Fission yeast Mus81•Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion. Genetics 165: 2289-2293 (2003) PMCID: PMC1462924 Abstract | pdf file

Taylor AF, Smith GR. RecBCD enzyme is a DNA helicase with fast and slow motors of opposite polarity. Nature 423: 889-893 (2003) Abstract | pdf file

Davis L, Smith GR. Non-random homolog segregation at meiosis I in Schizosaccharomyces pombe mutants lacking recombination. Genetics 163: 857-874 (2003) PMCID: PMC1462471 Abstract | pdf file

Amundsen SK, Smith GR. Interchangeable parts of the Escherichia coli recombination machinery. Cell 112 741-744 (2003) Abstract | pdf file


Lucius AL, Vindigni A, Gregorian R, Ali JA, Taylor AF, Smith GR, Lohman TM. DNA unwinding step-size of E. coli RecBCD helicase determined from single turnover chemical quenched-flow kinetic studies. J. Mol. Biol. 324: 409-428 (2002) Abstract | pdf file

Milac TI, Adler FR, Smith GR. Maximal power tests for detecting defects in meiotic recombination. Genetics 161: 1333-1337 (2002) PMCID: PMC1462193 Abstract | pdf file

Amundsen SA, Taylor AF, Smith GR. A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme. Genetics 161: 483-492 (2002) Abstract | pdf file

Farah JA, Hartsuiker E, Mizuno K, Ohta K, Smith GR. A 160-bp palindrome is a Rad50•Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombeGenetics 161: 461-468 (2002) PMCID: PMC1462081 Abstract | pdf file

Steiner WW, Schreckhise RW, Smith GR. Meiotic DNA breaks at the S. pombe recombination hot spot M26Mol. Cell 9: 847-855 (2002) Abstract | pdf file

Young JA, Schreckhise RW, Steiner WW, Smith GR. Meiotic recombination remote from prominent DNA break sites in S. pombeMol. Cell 9: 253-263 (2002) Abstract | pdf file

Smith GR. Chi sequences. Encyclopedia of Genetics, Miller JH, Brenner S (eds. in chief) Academic Press 325-328 (2002)


Mizuno K, Hasemi T, Ubukata T, Yamada T, Lehmann E, Kohli J, Watanabe Y, Iino Y, Yamamoto M,. Fox ME, Smith GR, Murofushi H, Shibata T, Ohta K. Counteracting regulation of chromatin remodeling at a fission yeast cAMP responsive element-related recombination hotspot by stress-activated protein kinase, cAMP-dependent kinase and meiosis regulators. Genetics 159: 1467-1478 (2001) Abstract

Smith, GR. Homologous recombination near and far from DNA breaks: Alternative roles and contrasting views. Ann. Rev. Genet. 35: 243-274 (2001) pdf file

Davis L, Smith GR. Meiotic recombination and chromosome segregation in S. pombeProc. Natl. Acad. Sci. USA 98: 8395-8402 (2001). PMCID: PMC37449. Abstract  | pdf file


Fox MF, Yamada T, Ohta K, Smith GR. A family of CRE-related DNA sequences with meiotic recombination hotspot activity in Schizosaccharomyces pombeGenetics 156: 59-68 (2000) Abstract

Cervantes MD, Farah JA, Smith GR. Meiotic DNA breaks associated with recombination in S. pombeMolec. Cell 5: 883-888 (2000) Abstract

Amundsen SK, Taylor AF, Smith GR. The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination and DNA repair. Proc. Natl. Acad. Sci. USA 97: 7399-7404 (2000) Abstract


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