Original Research

2025 | 2024 | 2023 | 2022 | 2021 | 2020 | 2019 | 2018 | 2017 | 2016 | 2015 |2014 | 2013 | 2012 | 2010 | 2009 | 2007 | 2006 | 2005 - 2001 | 1999 - 1994

2025

Popchock AR, Hedouin S, Mao Y, Asbury CL, Stergachis AB, Biggins S. Stable centromere association of the yeast histone variant Cse4 requires its essential N-terminal domain EMBO J 2025 Jan 14;. doi 10.1038/s44318-025-00345-5. PubMed PMID: 39809842.

2024

Barrero DJ, Wijeratne SS, Zhao X, Cunningham GF, Yan R, Nelson CR, Arimura Y, Funabiki H, Asbury CL, Yu Z, Subramanian R, Biggins S. Architecture of native kinetochores revealed by structural studies utilizing a thermophilic yeast. Curr Biol. 2024 Sep 9;34(17):3881-3893.e5. doi: 10.1016/j.cub.2024.07.036. Epub 2024 Aug 9. PubMed PMID: 39127048; PubMed Central PMCID: PMC11387133.

Larson JD, Heitkamp NA, Murray LE, Popchock AR, Biggins S, Asbury CL. Kinetochores grip microtubules with directionally asymmetric strength. J Cell Biol 6 January 2025; 224 (1): e202405176. doi: https://doi.org/10.1083/jcb.202405176

2023

Leeds BK, Kostello KF, Liu YY, Nelson CR, Biggins S, Asbury CL. Mechanical coupling coordinates microtubule growth. Elife. 2023 Dec 27;12. doi: 10.7554/eLife.89467. PMID: 38150374; PMCID: PMC10752587

Popchock AR, Larson JD, Dubrulle J, Asbury CL, Biggins S. Direct observation of coordinated assembly of individual native centromeric nucleosomes. EMBO J. 2023 Sep 4;42(17):e114534. doi: 10.15252/embj.2023114534. Epub 2023 Jul 20. PubMed PMID: 37469281; PubMed Central PMCID: PMC10476280.

2022

Lee CH, Biggins S. Microtubule integrity regulates budding yeast RAM pathway gene expression. Front Cell Dev Biol. 2022;10:989820. doi: 10.3389/fcell.2022.989820. eCollection 2022. PubMed PMID: 36172269; PubMed Central PMCID: PMC9511886.

de Regt, AK, Clark CJ, Asbury CL,  and Biggins, S. Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments. Nat Commun 13, 2152 (2022). https://doi.org/10.1038/s41467-022-29542-8.

Herman JA, Arora S, Carter L, Zhu J, Biggins S, and Paddison PJ. Functional dissection of human mitotic genes using CRISPR-Cas9 tiling screens. Genes Dev. 2022 Apr 1;36(7-8):495-510. doi: 10.1101/gad.349319.121. Epub 2022 Apr 28. PMID: 35483740.

Hedouin, S, Logsdon GA, Underwood JG, and Biggins S. A transcriptional roadblock protects yeast centromeres. Nucleic Acids Research, 2022;, gkac117, https://doi.org/10.1093/nar/gkac117

2021

Sarangapani KK, Koch LB, Nelson CR, Asbury CL, Biggins S*. Kinetochore-bound Mps1 regulates kinetochore-microtubule attachments via Ndc80 phosphorylation. J Cell Biol. 2021 Dec 6;220(12):e202106130. doi: 10.1083/jcb.202106130. PMCID: PMC8641409..

2020

Biggins S, Hartwell L, Toczyski D. Fifty years of cycling. Mol Biol Cell. 2020; Dec 15;31(26):2868-2870. doi: 10.1091/mbc.E20-07-0495. PMID: 33320706.

Herman JA, Miller MP, Biggins S*. chTOG is a conserved mitotic error correction factor. eLife. 2020 Dec 30;9:e61773. doi: 10.7554/eLife.61773. PMCID: PMC7773332.

Gutierrez A, Kim JO, Umbreit NT, Asbury CL, Davis TN, Miller MP, Biggins S. Cdk1 Phosphorylation of the Dam1 Complex Strengthens Kinetochore-Microtubule Attachments. Curr Biol. 2020; Nov; 16;30(22):4491-4499.e5. doi: 10.1016/j.cub.2020.08.054. PMCID: PMC7497780.

Chong, SY, Cutler, S, Lin, J, Tsai C, Tsai H, Biggins S, Tsukiyama T, Lo, Y, Kao CF. H3K4 methylation at active genes mitigates transcription-replication conflicts during replication stress. Nat Commun. 2020; Feb; 11(809). doi: 10.1038/s41467-020-14595-4.

2019

Miller MP, Evans R, Zelter A, Geyer EA, MacCross MJ, Rice LM, Davis TN, Asbury CL, Biggins S.* (2018). Kinetochore-associated Stu2 promotes chromosome biorientation in vivo. PLos Genet. 2019; Oct; 15(10): e1008423. doi: 10.1371/journal.pgen.1008423. PMCID: PMC6795502.

Koch LB, Opoku KN, Deng Y, Barber A, Littleton AJ, London N, Biggins S*, Asbury CL*. Autophosphorylation is sufficient to release Mps1 kinase from native kinetochores. Proc Natl Acad Sci USA. 2019; Aug 27;116(35):17355-17360. doi: 10.1073/pnas.1901653116. PMCID: PMC6717314.

Leontiou I, London, N, May KM, Ma Y, Grzesiak L, Medina-Pritchard B, Amin P, Jevaprakash AA, Biggins S, Hardwich KG. The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest. Curr Biol. Jul 22;29(14):2407-2414.e7. doi: 10.1016/j.cub.2019.06.011. PMCID: PMC6657678.

2018

deRegt AK, Asbury CL, Biggins S*. Tension on kinetochore substrates is insufficient to prevent Aurora-triggered detachment. BioRxiv [Preprint] September 13, 2018. Available from: https://doi.org/10.1101/415992.

Suzuki A*, Gupta A, Long SK, Evans R, Badger BL, Salmon ED, Biggins S*, Bloom K. A Kinesin-5, Cin8, Recruits Protein Phosphatase 1 to Kinetochores and Regulates Chromosome Segregation. Curr Biol. 2018; 28, 2697-2704. doi: 10.1016/j.cub.2018.08.038. PMCID: PMC6267807.

Lang J, Barber A, Biggins S*. An assay for de novo kinetochore assembly reveals a key role for the CENP-T pathway in budding yeast. eLife. 2018;7:e37819. doi: 10.7554/eLife.37819. PMCID: PMC6097842.

Geyer EA, Miller MP, Brautigam CA, Biggins S., Rice, LM*. Design principles of a microtubule polymerase. eLife. 2018;7:e34574. doi: 10.7554/eLife.34574. PMCID: PMC5999394.

Gupta A, Evans RK, Koch LB, Littleton AJ, Biggins S*. Purification of kinetochores from the budding yeast Saccharomyces cerevisiae. Methods Cell Biol. 2018; 144:349-370. doi: 10.1016/bs.mcb.2018.03.023. PMCID: PMC6205221.

2017

Tubman ES, Biggins S, Odde DJ*. Stochastic Modeling Yields a Mechanistic Framework for Spindle Attachment Error Correction in Budding Yeast Mitosis. Cell Syst. 2017 Jun 28;4(6):645-650.e5. doi: 10.1016/j.cels.2017.05.003. PMCID: PMC5533192.

Deyter GM, Hildebrand EM, Barber AD, Biggins S*. Histone H4 Facilitates the Proteolysis of the Budding Yeast CENP-ACse4 Centromeric Histone Variant. Genetics. 2017 Jan;205(1):113-124. doi: 10.1534/genetics.116.194027. PMCID: PMC5223497.

2016

Miller MP, Asbury CL*, Biggins S*. A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments. Cell. 2016 Jun 2;165(6):1428-39. doi: 10.1016/j.cell.2016.04.030. PMCID: PMC4892958.

Hildebrand EM, Biggins S*. Regulation of Budding Yeast CENP-A levels Prevents Misincorporation at Promoter Nucleosomes and Transcriptional Defects.  PLoS Genet. 2016 Mar 16;12(3):e1005930.doi: 10.1371/journal.pgen.105930. eCollection 2016. PMCID: PMC4794243.

2015

Biggins S*. Under Tension: Kinetochores and Basic Research .  Genetics. 200(3):681-682, https://doi.org/10.1534/genetics.115.178467

2014

London N, Biggins S*. Signalling dynamics in the spindle checkpoint response.  Nat rev mol Cell Biol. 2014 Nov; 15(11):736-47. doi: 10.1038/nrm3888. Epub 2014 Oct 10. Review. PubMed PMID: 25303117; PubMed Central PMCID: PMC4283840.

Umbreit NT, Miller MP, Tien JF, Ortola JC, Gui L, Lee KK, Biggins S, Asbury CL, Davis TN*. Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation.  Nat Commun. 2014 Sep 19;5:4951. doi: 10.1038/ncomms5951. PubMed PMID: 25236177; PubMed Central PMCID: PMC4197110.

Sarangapani KK, Duro E, Deng Y, Alves Fde L, Ye Q, Opoku KN, Ceto S, Rappsilber J, Corbett KD, Biggins S, Marston AL*, Asbury CL*. Sister kinetochores are mechanically fused during meiosis l in yeast.  Science. 2014 Oct 10;346(6206):248-51. doi: 10.1126/science.1256729. Epub 2014 Sep 11. PubMed PMID: 25213378; PubMed Central PMCID: PMC4226495.

Deyter GM, Biggins S*. The FACT complex interacts with the E3 ubiquitin ligase Psh1 to prevent ectopic localization of CENP-A. Genes Dev. 2014 Aug 15;28(16):1815-26. doi: 10.1101/gad.243113.114. PubMed PMID: 25128498; PubMed Central PMCID: PMC4197964.

Driver JW, Powers AF, Sarangapani KK, Biggins S, Asbury CL*. Measuring kinetochore-microtubule interaction in vitro. Methods Enzymol. 2014;540:321-37. doi: 10.1016/B978-0-12-397924-7.00018-2. PubMed PMID: 24402315; PubMed Central PMCID: PMC4106247.

London N, Biggins S*. Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint. Genes Dev. 2014 Jan 15;28(2):140-52. doi: 10.1101/grad.233700.113. Epub 2014 Jan 8. PubMed PMID: 24402315; PubMed Central PMCID: PMC3909788.

2013

Ng TM, Lenstra TL, Duggan N, Jiang S, Ceto S, Holstege FC, Dai J, Boeke JD, Biggins S*. Kinetochore function and chromosome segregation rely on critical residues in histones H3 and H4 in budding yeast. Genetics. 2013 Nov;195(3):795-807. doi: 10.1534/genetics.113.152082. Epub 2013 Sep 13. PubMed PMID: 24037263; PubMed Central PMCID: PMC3813865.

Biggins S*. The composition, functions, and regulation of the budding yeast kinetochore. Genetics. 2013 Aug;194(4):817-46. doi: 10.1534/genetics.112.145276. PubMed PMID: 23908374; PubMed Central PMCID: PMC3730914.

Akiyoshi B, Nelson CR, Biggins S*. The aurora B kinase promotes inner and outer kinetochore interactions in budding yeast. Genetics. 2013 Jul;194(3):785-9. doi: 10.1534/genetics.113.150839. Epub 2013 May 1. PubMed PMID: 23636741; PubMed Central PMCID: PMC3697981.

Sarangapani, KK, Akiyoshi, B, Duggan NM, Biggins S, Asbury CL*. Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms. PNAS. 2013;110(18):7282-7287. doi.org/10.1073/pnas.1220700110. PubMed PMID: 23589891; PubMed Central PMCID: PMC3645574.

Akiyoshi B, Nelson CR, Duggan N, Ceto S, Ranish JA, Biggins S*. The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein. PLoS Genet. 2013;9(2):e1003216. doi: 10.1371/journal.pgen.1003216. Epub 2013 Feb 7. PubMed PMID: 23408894; PubMed Central PMCID: PMC3567142.

2012

Gonen S, Akiyoshi B, Iadanza MG, Shi D, Duggan N, Biggins S*, Gonen T*. The structure of purified kinetochores reveals multiple microtubule-attachment sites. Nat Struct Mol Biol. 2012 Sep;19(9):925-9. doi: 10.1038/nsmb.2358. Epub 2012 Aug 12. PubMed PMID: 22885327; PubMed Central PMCID: PMC3443262. 
Science Spotlight

London N, Ceto S, Ranish JA, Biggins S*. Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores. Curr Biol. 2012 May 22;22(10):900-6. doi: 10.1016/j.cub.2012.03.052. Epub 2012 Apr 19. PubMed PMID: 22521787; PubMed Central PMCID: PMC3723133.

Unnikrishnan A, Akiyoshi B, Biggins S, Tsukiyama T*. An efficient purification system for native minichromosome from Saccharomyces cerevisiae. Methods Mol Biol. 2012;833:115-23. doi: 10.1007/978-1-61779-477-3_8. PubMed PMID: 22183591; PubMed Central PMCID: PMC3243952.

2010

Akiyoshi B, Biggins S*. Cdc14-dependent dephosphorylation of a kinetochore protein prior to anaphase in Saccharomyces cerevisiae. Genetics. 2010 Dec;186(4):1487-91. doi: 10.1534/genetics.110.123653. Epub 2010 Oct 5. PubMed PMID: 20923974; PubMed Central PMCID: PMC2998326.

Akiyoshi B, Sarangapani KK, Powers AF, Nelson CR, Reichow SL, Arellano-Santoyo H, Gonen T, Ranish JA, Asbury CL*, Biggins S*. Tension directly stabilizes reconstituted kinetochore-microtubule attachments. Nature. 2010 Nov 25;468(7323):576-9. doi: 10.1038/nature09594. PubMed PMID: 21107429; PubMed Central PMCID: PMC3108429. 
News and Views

Ranjitkar P, Press MO, Yi X, Baker R, MacCoss MJ, Biggins S. An E3 ubiquitin ligase prevents ectopic localization of the centromeric histone H3 variant via the centromere targeting domain. Mol Cell. 2010 Nov 12;40(3):455-64. doi: 10.1016/j.molcel.2010.09.025. PubMed PMID: 21070971; PubMed Central PMCID: PMC2995698.

2009

Akiyoshi B, Nelson CR, Ranish JA, Biggins S*. Quantitative proteomic analysis of purified yeast kinetochores identifies a PP1 regulatory subunit. Genes Dev. 2009 Dec 15;23(24):2887-99. doi: 10.1101/gad.1865909. Epub . PubMed PMID: 19948764; PubMed Central PMCID: PMC2800092. 
Perspective

Akiyoshi B, Nelson CR, Ranish JA, Biggins S*. Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae. Genetics. 2009 Dec;183(4):1591-5. doi: 10.1534/genetics.109.109041. Epub 2009 Oct 12. PubMed PMID: 19822728; PubMed Central PMCID: PMC2787442.

Ng TM, Waples WG, Lavoie BD, Biggins S*. Pericentromeric sister chromatid cohesion promotes kinetochore biorientation. Mol Biol Cell. 2009 Sep;20(17):3818-27. doi: 10.1091/mbc.E09-04-0330. Epub 2009 Jul 15. PubMed PMID: 19605555; PubMed Central PMCID: PMC2735481.
Incyte

Pinsky BA, Nelson CR, Biggins S*. Protein phosphatase 1 regulates exit from the spindle checkpoint in budding yeast. Curr Biol. 2009 Jul 28;19(14):1182-7. doi: 10.1016/j.cub.2009.06.043. Epub 2009 Jul 9. PubMed PMID: 19592248; PubMed Central PMCID: PMC2731492. 
Comment on paper

2007

Kotwaliwale, C. V., Frei, S. B., Stern, B. M, and S. Biggins*.  2007.  A pathway containing the Ipl1/aurora protein kinase and the spindle midzone protein Ase1 regulates yeast spindle assembly.  Dev Cell.  13(3) 433-445. PubMed PMID: 17765685; PubMed Central PMCID: PMC2679386.

Furuyama, S. and S. Biggins*.  2007.  Centromere Identity is Specified by a Single Centromeric Nucleosome in Budding Yeast.  PNAS.  104(37) 14706-14711. Abstract Full text

Collins, K. A., Camahort, R., Seidel, C., Gerton, J. L., and S. Biggins*.  2007.  The overexpression of a Saccharomyces cerevisiae centromeric histone H3 variant mutant protein leads to a defect in kinetochore biorientation.  Genetics.  175: 513–52. Abstract Full text

2006

Pinsky, B. A., C. Kung, Shokat, K. M, and S. Biggins*.  2006.  The Ipl1/Aurora protein kinase activates the spindle checkpoint by creating unattached kinetochores.  Nat Cell Biol.  8(1) 78-83. Abstract Full text

Pinsky, B. A., C. V. Kotwaliwale, S. Y. Tatsutani, Breed, C. A., and S. Biggins*.  2006.  Glc7/Protein Phosphatase-1 Regulatory Subunits can oppose the Ipl1/Aurora Protein Kinase by redistributing Glc7.  Mol Cell Biol.  26(7) 2648-60. Abstract Full text

Norden, C., Mendoza, M., Dobbelaere, J., Kotwaliwale, C. V., S. Biggins and Y. Barral.*  2006.  The NoCut pathway links abscission to proper function of the spindle midzone and prevents chromosome breakage by the cytokinetic machinery.  Cell.  125(1) 85-98. Abstract

2005 - 2001

Collins, K. A., Castillo, A. R., Tatsutani, S. Y., and S. Biggins*. 2005.  De novo kinetochore assembly requires the centromeric histone H3 variant.  Mol Biol Cell.  (16) 5649-60.

Collins, K. A., Furuyama, S. and S. Biggins*. 2004.  Proteolysis contributes to the exclusive centromere localization of the yeast Cse4/CENP-A histone H3 variant.  Curr Biol.  14(21): 1968-1927. Abstract Full text

Pinsky, B. A., Tatsutani, S. Y., Collins, K. A., and S. Biggins*. 2003.  An Mtw1 Complex Promotes Kinetochore Biorientation that is Monitored by the lpl1/Aurora Protein Kinase.  Dev. Cell.  5: 735-745. Abstract Full Text

Buvelot, S., Tatsutani, S. Y., Vermaak, D. and S. Biggins*. 2003.  The budding yeast Ipl1/Aurora protein kinase regulates mitotic microtubule disassembly.  J.  Cell Biol.  160:329-339.

Bhalla, N., Biggins S. and A. W. Murray*. 2002.  Mutation of YCS4, a budding yeast condensin subunit, affects mitotic and non-mitotic chromosome behavior.  Mol Biol Cell.  13:  632-645. Abstract Full Text

Biggins, S.* and A. W. Murray.  2001.  The budding yeast protein kinase Ipl1/aurora allows the absence of tension to activate the spindle checkpoint.  Genes Dev.  15:3118-3129. Abstract Full Text

Biggins, S.*, N. Bhalla, A. Chang, D. Smith and A. W. Murray.  2001.  Genes Involved in Sister Chromatid Separation and Segregation in the Budding Yeast Saccharomyces cerevisiae.  Genetics.  159:453-470. Abstract Full Text

1999 - 1994

Biggins, S.*, F. F. Severin, N. Bhalla, I. Sassoon, A. A. Hyman and A. W. Murray.  1999.  The conserved protein kinase Ipl1 regulates microtubule binding to kinetochores in budding yeast.  Genes Dev.  13:532-544. Abstract Full Text

Sullivan, D. S., S. Biggins and M. D. Rose.*  1998.  The yeast centrin, Cdc31p, and the interacting kinase, Kic1p, are required for cell integrity.  J. Cell Biol.  143:737-749.

Biggins, S., I. Ivanovska and M. D. Rose.*  1996.  Yeast ubiquitin-like proteins are involved in duplication of the microtubule organizing center.  J Cell Biol.  133:1331-1346. 

Biggins, S. and M. D. Rose.*  1994.  Direct interaction between spindle pole body components; Kar1p is required for Cdc31p localization to the spindle pole body.  J. Cell Biol.  125:843-852.

Invited Reviews

S. Biggins*.  2013. The Composition, Functions and Regulation of the Budding Yeast Kinetochore. Genetics. 194(4):817-46. PMCID: PMC3730914.

Akiyoshi, B. and S. Biggins*. 2012.  Reconstituting the kinetochore-microtubule interface:  what, why and how. Chromosoma.  DOI 10.1007/s00412-012-0362-0.  PMCID: PMC3383909.

Kotwaliwale, C. V. and S. Biggins*. 2009.  Post-translational modifications that regulate kinetochore activity.  The Kinetochore: from Molecular Discoveries to Cancer Therapy. Eds. De Wulf, P. and Earnshaw, W.C.  Springer Publ., New York City.

Kotwaliwale, C. V., and S. Biggins*. 2006.  Microtubule capture: A concerted effort.  Cell. 127 1105-1108. Abstract Full text

Pinsky, B. A. and S. Biggins*. 2005.  The spindle checkpoint: tension vs. attachment.  Trends Cell Biol.  (15) 486-493.

Kamakaka, R.* and S. Biggins*. 2004.  Histone variants: deviants?  Genes Dev.  19 (3) 294-310. Abstract Full text

Biggins, S*. 2004.  Correcting SYNful attachments.  Nature Cell Biol.  6(3):  181-183. Abstract Full text

Biggins, S.* and C. E. Walczak*.  2003.  Captivating Capture:  How Microtubules Attach to Kinetochores.  Curr Biol.  13: R449-R460. Abstract Full Text

Pinksy, B. A. and S. Biggins.*  2003.  Top SUMO wrestles centromeric cohesion.  Dev. Cell.  3:4-6. Abstract Full Text

Biggins, S.* and A. W. Murray. 1998. Sister chromatid cohesion in mitosis. Curr. Op. Cell Biol.  10:769-775.  Updated version 1999.  Curr. Op. Genet. Dev.  9:230-6. Abstract Full Text

Rose, M. D.*, S. Biggins and L. L. Satterwhite.  1993.  Unraveling the tangled web at the microtubule organizing center.  Curr. Op. In Cell Biol.  5:105-115.