Standard Operating Protocols

Collection of samples for proteomic analysis

• SOP #1: Collection of frozen tissue samples for proteomic analysis
• SOP #2: Collection of Peripheral Blood by venipuncture, Labeling, Processing and Storage for proteomic analysis  (UNDER REVISION)
• SOP #3: Collection of Blood from a port for proteomic analysis (UNDER REVISION)
• SOP #4: Processing blood samples to isolate plasma for proteomic analysis 

PBMC extraction from whole blood

• SOP #5: Preparation of PBMC from whole blood by Ficoll gradient
• SOP #6: Preparation of PBMC from whole blood by CPT mononuclear preparation tube

Preparation of protein lysates for proteomics

• SOP #7: Preparation of protein lysates from frozen tumor samples for mass spectrometry
• SOP #8: Preparation of protein lysates from FFPE tumor samples for mass spectrometry
• SOP #9: Preparation of protein lysates from cultured cells for mass spectrometry