Typical Protein Yields

Biospecimen Estimated protein yield
HeLa cell line 200 µg / 106 cells
MCF10A cell line 300 µg / 106 cells
HMEC line 200 µg / 106 cells
SKBR3 cell line 200 µg / 106 cells 
MDA-MB-361 cell line 160 µg / 106 cells
MDA-MB-468 cell line 220 µg / 106 cells
BT474 cell line 350 µg / 106 cells
HCC1954 cell line 300 µg / 106 cells
lymphoblast cell line (LBL) 100 µg / 106 cells
PBMC (Ficoll preparation) 65 µg protein / 106 cells (~1mL whole blood)
PBMC (expanded in culture) 25 µg / 106 cells
AML blasts  18 µg / 106 cells 
Frozen tumor biopsy (approximate size of a pencil eraser)  1-2 mg
Frozen tumor biopsy (approximate size of a wet grain of rice) 300-500 ug
Breast tumor tissue (fresh frozen) 20-50 µg /mg wet wt. input (2.0-5.0% yield) 
Ovarian tumor tissue (fresh frozen)  25-45 µg /mg wet wt. input (2.5-4.5% yield)  
Pediatric brain tumor tissue (fresh frozen) 30-65 µg /mg wet wt. input (3.0-6.5% yield) 
Brain tumor tissue (fresh frozen) 50-70 µg /mg wet wt. input (5.0-7.0% yield)
Endometrium / Uterine tumor tissue (fresh frozen) 45-75 µg /mg wet wt. input (4.5-7.5% yield)
Head and Neck tumor tissue (fresh frozen) 20-70 µg /mg wet wt. input (2.0-7.0% yield)
Kidney tumor tissue (fresh frozen) 35-70 µg /mg wet wt. input (3.5-7.0% yield)
Lung-Adeno tumor tissue (fresh frozen) 40-65 µg /mg wet wt. input (4.0-6.5% yield)
Lung-Squamous tumor tissue (fresh frozen) 40-70 µg /mg wet wt. input (4.0-7.0% yield)
Pancreas tumor tissue (fresh frozen) 30-40 µg /mg wet wt. input (3.0-4.0% yield)
Soft Tissue / Sarcoma tumor tissue (fresh frozen) 30-45 µg /mg wet wt. input (3.0-4.5% yield)
FFPE section (1cm x 1cm x 10µm) 10-50 µg 
Lymph node FFPE (5µm section)  13-68 µg  
Laser Capture Microdissected tissue 3-5 µg / 10,000 cells 
Core Needle Biopsy    (16G x 1 cm) 100-200 µg
Core Needle Biopsy    (18G x 1 cm) 50-100 µg
Core Needle Biopsy    (20G x 1 cm) 30-60 µg
Core Needle Biopsy    (21G x 1 cm) 20-40 µg