Typical Protein Yields
| Biospecimen | Estimated protein yield |
| HeLa cell line | 200 µg / 106 cells |
| MCF10A cell line | 300 µg / 106 cells |
| HMEC line | 200 µg / 106 cells |
| SKBR3 cell line | 200 µg / 106 cells |
| MDA-MB-361 cell line | 160 µg / 106 cells |
| MDA-MB-468 cell line | 220 µg / 106 cells |
| BT474 cell line | 350 µg / 106 cells |
| HCC1954 cell line | 300 µg / 106 cells |
| lymphoblast cell line (LBL) | 100 µg / 106 cells |
| PBMC (Ficoll preparation) | 40 µg protein / 106 cells (~1mL whole blood) |
| PBMC (expanded in culture) | 25 µg / 106 cells |
| AML blasts | 18 µg / 106 cells |
| Frozen tumor biopsy (approximate size of a pencil eraser) | 1-2 mg |
| Frozen tumor biopsy (approximate size of a wet grain of rice) | 300-500 ug |
| Breast tumor tissue (fresh frozen) | 20-50 µg /mg wet wt. input (2.0-5.0% yield) |
| Ovarian tumor tissue (fresh frozen) | 25-45 µg /mg wet wt. input (2.5-4.5% yield) |
| Pediatric brain tumor tissue (fresh frozen) | 30-65 µg /mg wet wt. input (3.0-6.5% yield) |
| Brain tumor tissue (fresh frozen) | 50-70 µg /mg wet wt. input (5.0-7.0% yield) |
| Endometrium / Uterine tumor tissue (fresh frozen) | 45-75 µg /mg wet wt. input (4.5-7.5% yield) |
| Head and Neck tumor tissue (fresh frozen) | 20-70 µg /mg wet wt. input (2.0-7.0% yield) |
| Kidney tumor tissue (fresh frozen) | 35-70 µg /mg wet wt. input (3.5-7.0% yield) |
| Lung-Adeno tumor tissue (fresh frozen) | 40-65 µg /mg wet wt. input (4.0-6.5% yield) |
| Lung-Squamous tumor tissue (fresh frozen) | 40-70 µg /mg wet wt. input (4.0-7.0% yield) |
| Pancreas tumor tissue (fresh frozen) | 30-40 µg /mg wet wt. input (3.0-4.0% yield) |
| Soft Tissue / Sarcoma tumor tissue (fresh frozen) | 30-45 µg /mg wet wt. input (3.0-4.5% yield) |
| FFPE section (1 cm x 1 cm x 10 µm) | 10-50 µg |
| 1 mm diameter x 1-4 mm deep punch from FFPE block | 25 µg – 100 µg |
| 2 mm diameter x 1-4 mm deep punch from FFPE block | 50 µg – 275 µg |
| Lymph node FFPE (5 µm section) | 13-68 µg |
| Laser Capture Microdissected tissue | 3-5 µg / 10,000 cells |
| Core Needle Biopsy (16G x 1 cm) | 100-200 µg |
| Core Needle Biopsy (18G x 1 cm) | 50-100 µg |
| Core Needle Biopsy (20G x 1 cm) | 30-60 µg |
| Core Needle Biopsy (21G x 1 cm) | 20-40 µg |
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© 2023 Fred Hutchinson Cancer Center, a 501(c)(3) nonprofit organization.