FFPE Specimens | Fresh Frozen Specimens | Blood-Derived Cells | Cultured Cells
Note: Protein yield estimates are based on historical extraction data from similar specimens. Biological variability, including differences in tissue composition, disease state, treatment effects, and specimen handling, can influence protein recovery. The values below should be interpreted as approximate expectations rather than guaranteed yields.
Biospecimen | Estimated protein yield |
FFPE biopsy section (1 cm x 1 cm x 10 µm) | 10-50 µg |
1 mm diameter x 1-4 mm deep punch from FFPE block | 25 µg – 100 µg |
2 mm diameter x 1-4 mm deep punch from FFPE block | 50 µg – 275 µg |
Lymph node FFPE (5 µm section) | 13-68 µg |
Laser capture microdissected tissue | 3-5 µg / 10,000 cells |
Core needle biopsy, FFPE (16G x 1 cm) | 100-200 µg |
Core needle biopsy, FFPE (18G x 1 cm) | 50-100 µg |
Core needle biopsy, FFPE (20G x 1 cm) | 30-60 µg |
Core needle biopsy, FFPE (21G x 1 cm) | 20-40 µg |
Biospecimen | Estimated protein yield |
Frozen tumor biopsy (approximate size of a pencil eraser) | 1-2 mg |
Frozen tumor biopsy (approximate size of a wet grain of rice) | 300-500 ug |
Breast tumor tissue (fresh frozen) | 20-50 µg /mg wet wt. input (2.0-5.0% yield) |
Ovarian tumor tissue (fresh frozen) | 25-45 µg /mg wet wt. input (2.5-4.5% yield) |
Pediatric brain tumor tissue (fresh frozen) | 30-65 µg /mg wet wt. input (3.0-6.5% yield) |
Brain tumor tissue (fresh frozen) | 50-70 µg /mg wet wt. input (5.0-7.0% yield) |
Endometrium / Uterine tumor tissue (fresh frozen) | 45-75 µg /mg wet wt. input (4.5-7.5% yield) |
Head and Neck tumor tissue (fresh frozen) | 20-70 µg /mg wet wt. input (2.0-7.0% yield) |
Kidney tumor tissue (fresh frozen) | 35-70 µg /mg wet wt. input (3.5-7.0% yield) |
Lung-Adeno tumor tissue (fresh frozen) | 40-65 µg /mg wet wt. input (4.0-6.5% yield) |
Lung-Squamous tumor tissue (fresh frozen) | 40-70 µg /mg wet wt. input (4.0-7.0% yield) |
Pancreas tumor tissue (fresh frozen) | 30-40 µg /mg wet wt. input (3.0-4.0% yield) |
Soft Tissue / Sarcoma tumor tissue (fresh frozen) | 30-45 µg /mg wet wt. input (3.0-4.5% yield) |
Core needle biopsy, fresh frozen (16G x 1 cm) | 200-500 µg |
Core needle biopsy, fresh frozen (18G x 1 cm) | 100-250 µg |
Core needle biopsy, fresh frozen (20G x 1 cm) | 60-150 µg |
Core needle biopsy, fresh frozen (21G x 1 cm) | 40-100 µg |
Biospecimen | Estimated protein yield |
PBMC (Ficoll preparation) | 65 µg protein / 106 cells (~1mL whole blood) |
PBMC (expanded in culture) | 25 µg / 106 cells |
AML blasts | 18 µg / 106 cells |
Biospecimen | Estimated protein yield |
HeLa cell line | 200 µg / 106 cells |
MCF10A cell line | 300 µg / 106 cells |
HMEC line | 200 µg / 106 cells |
SKBR3 cell line | 200 µg / 106 cells |
MDA-MB-361 cell line | 160 µg / 106 cells |
MDA-MB-468 cell line | 220 µg / 106 cells |
BT474 cell line | 350 µg / 106 cells |
HCC1954 cell line | 300 µg / 106 cells |
lymphoblast cell line (LBL) | 100 µg / 106 cells |