Biospecimen

Estimated protein yield

FFPE biopsy section (1 cm x 1 cm x 10 µm)

10-50 µg 

1 mm diameter x 1-4 mm deep punch from FFPE block

25 µg – 100 µg

2 mm diameter x 1-4 mm deep punch from FFPE block

50 µg – 275 µg

Lymph node FFPE (5 µm section) 

13-68 µg  

Laser capture microdissected tissue

3-5 µg / 10,000 cells 

Core needle biopsy, FFPE  (16G x 1 cm)

100-200 µg

Core needle biopsy, FFPE  (18G x 1 cm)

50-100 µg

Core needle biopsy, FFPE  (20G x 1 cm)

30-60 µg

Core needle biopsy, FFPE  (21G x 1 cm)

20-40 µg

Biospecimen

Estimated protein yield

Frozen tumor biopsy (approximate size of a pencil eraser) 

1-2 mg

Frozen tumor biopsy (approximate size of a wet grain of rice)

300-500 ug

Breast tumor tissue (fresh frozen)

20-50 µg /mg wet wt. input (2.0-5.0% yield) 

Ovarian tumor tissue (fresh frozen) 

25-45 µg /mg wet wt. input (2.5-4.5% yield)  

Pediatric brain tumor tissue (fresh frozen)

30-65 µg /mg wet wt. input (3.0-6.5% yield) 

Brain tumor tissue (fresh frozen)

50-70 µg /mg wet wt. input (5.0-7.0% yield)

Endometrium / Uterine tumor tissue (fresh frozen)

45-75 µg /mg wet wt. input (4.5-7.5% yield)

Head and Neck tumor tissue (fresh frozen)

20-70 µg /mg wet wt. input (2.0-7.0% yield)

Kidney tumor tissue (fresh frozen)

35-70 µg /mg wet wt. input (3.5-7.0% yield)

Lung-Adeno tumor tissue (fresh frozen)

40-65 µg /mg wet wt. input (4.0-6.5% yield)

Lung-Squamous tumor tissue (fresh frozen)

40-70 µg /mg wet wt. input (4.0-7.0% yield)

Pancreas tumor tissue (fresh frozen)

30-40 µg /mg wet wt. input (3.0-4.0% yield)

Soft Tissue / Sarcoma tumor tissue (fresh frozen)

30-45 µg /mg wet wt. input (3.0-4.5% yield)

Core needle biopsy, fresh frozen  (16G x 1 cm)

200-500 µg

Core needle biopsy, fresh frozen  (18G x 1 cm)

100-250 µg

Core needle biopsy, fresh frozen  (20G x 1 cm)

60-150 µg

Core needle biopsy, fresh frozen  (21G x 1 cm)

40-100 µg

Biospecimen

Estimated protein yield

PBMC (Ficoll preparation)

40 µg protein / 10⁶ cells (~1mL whole blood)

PBMC (expanded in culture)

25 µg / 10⁶ cells

AML blasts 

18 µg / 10⁶ cells 

Biospecimen

Estimated protein yield

HeLa cell line

200 µg / 10⁶ cells

MCF10A cell line

300 µg / 10⁶ cells

HMEC line

200 µg / 10⁶ cells

SKBR3 cell line

200 µg / 10⁶ cells 

MDA-MB-361 cell line

160 µg / 10⁶ cells

MDA-MB-468 cell line

220 µg / 10⁶ cells

BT474 cell line

350 µg / 10⁶ cells

HCC1954 cell line

300 µg / 10⁶ cells

lymphoblast cell line (LBL)

100 µg / 10⁶ cells