Biospecimen |
Estimated protein yield |
HeLa cell line |
200 µg / 106 cells |
MCF10A cell line |
300 µg / 106 cells |
HMEC line |
200 µg / 106 cells |
SKBR3 cell line |
200 µg / 106 cells |
MDA-MB-361 cell line |
160 µg / 106 cells |
MDA-MB-468 cell line |
220 µg / 106 cells |
BT474 cell line |
350 µg / 106 cells |
HCC1954 cell line |
300 µg / 106 cells |
lymphoblast cell line (LBL) |
100 µg / 106 cells |
PBMC (Ficoll preparation) |
40 µg protein / 106 cells (~1mL whole blood) |
PBMC (expanded in culture) |
25 µg / 106 cells |
AML blasts |
18 µg / 106 cells |
Frozen tumor biopsy (approximate size of a pencil eraser) |
1-2 mg |
Frozen tumor biopsy (approximate size of a wet grain of rice) |
300-500 ug |
Breast tumor tissue (fresh frozen) |
20-50 µg /mg wet wt. input (2.0-5.0% yield) |
Ovarian tumor tissue (fresh frozen) |
25-45 µg /mg wet wt. input (2.5-4.5% yield) |
Pediatric brain tumor tissue (fresh frozen) |
30-65 µg /mg wet wt. input (3.0-6.5% yield) |
Brain tumor tissue (fresh frozen) |
50-70 µg /mg wet wt. input (5.0-7.0% yield) |
Endometrium / Uterine tumor tissue (fresh frozen) |
45-75 µg /mg wet wt. input (4.5-7.5% yield) |
Head and Neck tumor tissue (fresh frozen) |
20-70 µg /mg wet wt. input (2.0-7.0% yield) |
Kidney tumor tissue (fresh frozen) |
35-70 µg /mg wet wt. input (3.5-7.0% yield) |
Lung-Adeno tumor tissue (fresh frozen) |
40-65 µg /mg wet wt. input (4.0-6.5% yield) |
Lung-Squamous tumor tissue (fresh frozen) |
40-70 µg /mg wet wt. input (4.0-7.0% yield) |
Pancreas tumor tissue (fresh frozen) |
30-40 µg /mg wet wt. input (3.0-4.0% yield) |
Soft Tissue / Sarcoma tumor tissue (fresh frozen) |
30-45 µg /mg wet wt. input (3.0-4.5% yield) |
FFPE section (1 cm x 1 cm x 10 µm) |
10-50 µg |
1 mm diameter x 1-4 mm deep punch from FFPE block |
25 µg – 100 µg |
2 mm diameter x 1-4 mm deep punch from FFPE block |
50 µg – 275 µg |
Lymph node FFPE (5 µm section) |
13-68 µg |
Laser Capture Microdissected tissue |
3-5 µg / 10,000 cells |
Core Needle Biopsy (16G x 1 cm) |
100-200 µg |
Core Needle Biopsy (18G x 1 cm) |
50-100 µg |
Core Needle Biopsy (20G x 1 cm) |
30-60 µg |
Core Needle Biopsy (21G x 1 cm) |
20-40 µg |