Adair Lab

2020 | 2019 | 2018 | 2017 | 2016 | 2012 | 2011 | 2010 | 2009 | 2007 | 2005 | 2004

2020

Adair JE, Enstrom MR, Haworth KG, Schefter LE, Shahbazi R, Humphrys DR, Porter S, Tam K, Porteus MH, Kiem HP. DNA Barcoding in Nonhuman Primates Reveals Important Limitations in Retrovirus Integration Site Analysis. Mol. Ther. Methods Clin. Dev. 2020, 17:796-809. PMCID: PMC7184234.

2019

Peterson CW ^†, Adair JE ^†, Wohlfahrt ME, Deleage C, Radtke S, Norman KK, Norgaard ZK, Schefter LE, Sghia-Hughes G, Repetto A, Baldessari A, Murnane RD, Estes JD, Kiem HP. Autologous, gene-modified hematopoietic stem and progenitor cells repopulate central nervous system with distinct clonal variants. Stem Cell Reports. 2019, 13(1): 91-104. PMCID: PMC6626873.

Shahbazi R, Humbert O, Haworth KG, Sghia-Hughes G, Kiem HP, Adair JE  Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations. Nat Mater. 2019, 18(10):1124-1132. PMCID: PMC6754292.

2018

Adair JE, Chandrasekaran D, Sghia-Hughes G, Haworth KG, Woolfrey AE, Burroughs LM, Choi GY, Becker PS, Kiem HP. Novel lineage depletion preserves autologous blood stem cells for gene therapy of Fanconi Anemia complementation group A. Haematologica. 2018, 103(11):1806-1814. PMCID: PMC6278989

Turtle CJ, Adair JE. Driving CARs Across New Borders. Human gene therapy. 2018; 29(5):529. PubMed [journal] PMID: 29715075

Adair JE. An Interview with Michel Sadelain, MD, PhD. Human gene therapy. 2018; 29(5):530-533. PubMed [journal] PMID: 29715074

2017

Radtke S†, Adair JE†, Giese MA, Chan YY, Norgaard ZK, Enstrom M, Haworth KG, Schefter LE, Kiem HP. A distinct hematopoietic stem/progenitor cell population for rapid multilineage engraftment. Sci. Transl. Med. 2017, 9(414), eaan 1145. PMCID: PMC6467214

Adair JE, Sevilla J, Heredia CD, Becker PS, Kiem HP, Bueren J. Lessons Learned from Two Decades of Clinical Trial Experience in Gene Therapy for Fanconi Anemia. Current gene therapy. 2017; 16(5):338-348. PubMed [journal] PMID: 28103787

2016

Adair JE, Waters T, Haworth KG, Kubek SP, Trobridge GD, Hocum JD, Heimfeld S,Kiem HP. Semi-automated closed system manufacturing of lentivirus gene-modified haematopoietic stem cells for gene therapy. Nature communications. 2016; 7:13173. PubMed [journal] PMID: 27762266, PMCID: PMC5080442

2014

Adair JE, Johnston SK, Mrugala MM, Beard BC, Guyman LA, Baldock AL, Bridge CA, Hawkins-Daarud A, Gori JL, Born DE, Gonzalez-Cuyar LF, Silbergeld DL, Rockne RC,  Storer BE, Rockhill JK, Swanson KR, Kiem HP. Gene therapy enhances chemotherapy tolerance and efficacy in glioblastoma patients. The Journal of clinical investigation. 2014; 124(9):4082-92. PubMed [journal] PMID: 25105369, PMCID: PMC4151207

Adair JE, Weitzman MD. Applying the speed-dating model and other approaches to foster future leaders for the American Society of Gene and Cell Therapy. Molecular therapy : the journal of the American Society of Gene Therapy. 2014; 22(8):1397-1398. PubMed [journal] PMID: 25082084, PMCID: PMC4435583

O'Reilly M, Federoff HJ, Fong Y, Kohn DB, Patterson AP, Ahmed N, Asokan A, Boye SE, Crystal RG, De Oliveira S, Gargiulo L, Harper SQ, Ikeda Y, Jambou R, Montgomery M, Prograis L, Rosenthal E, Sterman DH, Vandenberghe LH, Zoloth L, Abedi M, Adair J, Adusumilli PS, Goins WF, Gray J, Monahan P, Popplewell L, Sena-Esteves M, Tannous B, Weber T, Wierda W, Gopal-Srivastava R, McDonald CL, Rosenblum D, Corrigan-Curay J. Gene therapy: charting a future course--summary of a National Institutes of Health Workshop, April 12, 2013. Human gene therapy. 2014; 25(6):488-97. PubMed [journal] PMID: 24773122, PMCID: PMC4064731

Beard BC, Adair JE, Trobridge GD, Kiem HP. High-throughput genomic mapping of vector integration sites in gene therapy studies. Methods in molecular biology (Clifton, N.J.). 2014; 1185:321-44. PubMed [journal] PMID: 25062639

2012

Adair JE, Zhao X, Chien S, Fang M, Wohlfahrt ME, Trobridge GD, Taylor JA, Beard BC, Kiem HP, Becker PS. Cyclophosphamide promotes engraftment of gene-modified cells in a mouse model of Fanconi anemia without causing cytogenetic abnormalities. Journal of molecular medicine (Berlin, Germany). 2012; 90(11):1283-94. NIHMSID: NIHMS461642 PubMed [journal] PMID: 22660274, PMCID: PMC3650894

Mrugala MM, Adair J, Kiem HP. Chemoprotection in glioblastoma therapy: reality or a dream? CNS oncology. 2012; 1(1):11-4. PubMed [journal] PMID: 25054293

Adair JE, Beard BC, Trobridge GD, Neff T, Rockhill JK, Silbergeld DL, Mrugala MM, Kiem HP. Extended survival of glioblastoma patients after chemoprotective HSC gene therapy. Science translational medicine. 2012; 4(133):133ra57. NIHMSID: NIHMS461629 PubMed [journal] PMID: 22572881, PMCID: PMC3650895

Mrugala MM, Adair JE, Kiem HP. Outside the box--novel therapeutic strategies for  glioblastoma. Cancer journal (Sudbury, Mass.). 2012; 18(1):51-8. PubMed [journal] PMID: 22290258

2011

Tolar J, Adair JE, Antoniou M, Bartholomae CC, Becker PS, Blazar BR, Bueren J, Carroll T, Cavazzana-Calvo M, Clapp DW, Dalgleish R, Galy A, Gaspar HB, Hanenberg H, Von Kalle C, Kiem HP, Lindeman D, Naldini L, Navarro S, Renella R, Rio P, Sevilla J, Schmidt M, Verhoeyen E, Wagner JE, Williams DA, Thrasher AJ. Stem cell gene therapy for fanconi anemia: report from the 1st international Fanconi anemia gene therapy working group meeting. Molecular therapy : the journal of the American Society of Gene Therapy. 2011; 19(7):1193-8. PubMed [journal] PMID: 21540837, PMCID: PMC3129570

2010

Mrugala MM, Adair J, Kiem HP. Temozolomide: Expanding its role in brain cancer. Drugs of today (Barcelona, Spain : 1998). 2010; 46(11):833-46. PubMed [journal] PMID: 21225022

Becker PS, Taylor JA, Trobridge GD, Zhao X, Beard BC, Chien S, Adair J, Kohn DB,  Wagner JE, Shimamura A, Kiem HP. Preclinical correction of human Fanconi anemia complementation group A bone marrow cells using a safety-modified lentiviral vector. Gene therapy. 2010; 17(10):1244-52. NIHMSID: NIHMS182086 PubMed [journal] PMID: 20485382, PMCID: PMC2927804

Beard BC, Trobridge GD, Ironside C, McCune JS, Adair JE, Kiem HP. Efficient and stable MGMT-mediated selection of long-term repopulating stem cells in nonhuman primates. The Journal of clinical investigation. 2010; 120(7):2345-54. PubMed [journal] PMID: 20551514, PMCID: PMC2898586

Collaborations

2020 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2012 | 2011 | 2010

2020

Rust BJ, Becker PS, Chandrasekaran D, Kubek S, Peterson CW, Adair JE, Kiem HP. Induction of VSV-G-specific adaptive immunity following transplantation of autologous lineage-depleted hematopoietic stem cells modified with VSV-enveloped lentivirus in the absence of conditioning. Mol. Ther. Methods Clin. Dev. 2020. [in press].

Radtke S, Pande D, Cui M, Perez AM, Chan YY, Enstrom M, Schmuck S, Berger A, Eunson T, Adair JE, Kiem HP. Purification of human CD34+CD90+ cell reduces target cell population and improves lentiviral transduction for gene therapy. Mol. Ther. Methods Clin. Dev. 2020. [in press].

Sheih A, Voillet V, Hanafi LA, DeBerg HA, Yajima M, Hawkins R, Gersuk V, Riddell SR, Maloney DG, Wohlfahrt ME, Pande D, Enstrom MR, Kiem HP, Adair JE, Gottardo R, Linsley PS, Turtle CJ. Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy. Nat Commun. 2020, 11(1):219. PMCID: PMC6954177

2019

Humbert O, Radtke S, Samuelson C, Carrillo RR, Perez AM, Reddy SS, Lux C, Pattabhi S, Schefter LE, Negre O, Lee CM, Bao G, Adair JE, Peterson CW, Rawlings DJ, Scharenberg AM, Kiem HP. Therapeutically relevant engraftment of a CRISPR-Cas9-edited HSC-enriched population with HbF reactivation in nonhuman primates. Sci. Transl. Med. 2019, 11(503). PMID: 31366580

Ozog S, Timberlake ND, Hermann K, Garijo O, Haworth KG, Shi G, Glinkerman CM, Schefter LE, D'Souza S, Simpson E, Sghia-Hughes G, Carillo RR, Boger DL, Kiem HP, Slukvin I, Ryu BY, Sorrentino BP, Adair JE, Snyder SA, Compton AA, Torbett BE. Resveratrol timer enhances gene delivery to hematopoietic stem cells by reducing antiviral restriction at endosomes. Blood. 2019, 134(16):1298-1311. PMCID: PMC6888140

2018

Haworth KG, Ironside C, Ramirez MA, Weitz S, Beard BC, Schwartz JD, Adair JE, Kiem HP. Minimal conditioning in Fanconi anemia promotes multi-lineage marrow engraftment at 10-fold lower cell doses. J. Gene Med. 2018, 20(10-11):e3050. PMID: 30129972

Humbert O, Chan F, Rajawat YS, Torgerson TR, Burtner CR, Hubbard NW, Humphrys D,  Norgaard ZK, O'Donnell P, Adair JE, Trobridge GD, Scharenberg AM, Felsburg PJ, Rawlings DJ, Kiem HP. Rapid immune reconstitution of SCID-X1 canines after G-CSF/AMD3100 mobilization and in vivo gene therapy. Blood advances. 2018;2(9):987-999. PubMed [journal] PMID: 29720491, PMCID: PMC5942001

Ngom M, Imren S, Maetzig T, Adair JE, Knapp DJHF, Chagraoui J, Fares I, Bordeleau ME, Saubageau G, Leboulch P, Eaves C, Humphries RK. UM171 enhances lentiviral gene transfer and recovery of primitive human hematopoietic cells. Mol. Ther. Meth. Clin. Dev. 2018, 10:156-164. PMCID: PMC6077133

Haworth KG, Schefter LE, Norgaard ZK, Ironside C, Adair JE, Kiem HP. HIV infection results in clonal expansions containing integrations with pathogenesis-related biological pathways. J. Clin Inves. Insights. 2018, 3(13). PMCID: PMC6124524

2017

Haworth KG, Ironside C, Norgaard ZK, Obenza WM, Adair JE, Kiem HP. In vivo murine-matured human CD3+ cells as a preclinical model for T cell-based immunotherapies. Mol. Ther. Methods Clin. Dev. 2017, 6:17-30. PMCID: PMC5470556

Singh S, Khan I, Khim S, Seymour B, Sommer K, Wielgosz M, Norgaard Z, Kiem HP, Adair J, Liggitt D, Nienhuis A, Rawlings DJ. Safe and Effective Gene Therapy for  Murine Wiskott-Aldrich Syndrome Using an Insulated Lentiviral Vector. Molecular therapy. Methods & clinical development. 2017; 4:1-16. PubMed [journal] PMID: 28344987, PMCID: PMC5363182

Gori JL, Butler JM, Kunar B, Poulos MG, Ginsberg M, Nolan DJ, Norgaard ZK, Adair JE, Rafii S, Kiem HP. Endothelial Cells Promote Expansion of Long-Term Engrafting Marrow Hematopoietic Stem and Progenitor Cells in Primates. Stem cells translational medicine. 2017; 6(3):864-876. PubMed [journal] PMID: 28297579, PMCID: PMC5442761

2016

Peterson CW, Haworth KG, Burke BP, Polacino P, Norman KK, Adair JE, Hu SL, Bartlett JS, Symonds GP, Kiem HP. Multilineage polyclonal engraftment of Cal-1 gene-modified cells and in vivo selection after SHIV infection in a nonhuman primate model of AIDS. Molecular therapy. Methods & clinical development. 2016; 3:16007. PubMed [journal] PMID: 26958575, PMCID: PMC4765711

2015

Olszko ME, Adair JE, Linde I, Rae DT, Trobridge P, Hocum JD, Rawlings DJ, Kiem HP, Trobridge GD. Foamy viral vector integration sites in SCID-repopulating cellsafter MGMTP140K-mediated in vivo selection. Gene therapy. 2015; 22(7):591-5. NIHMSID: NIHMS693568 PubMed [journal] PMID: 25786870, PMCID: PMC4490015

Burtner CR, Chandrasekaran D, Santos EB, Beard BC, Adair JE, Hamlin DK, Wilbur DS, Sandmaier BM, Kiem HP. (211)Astatine-Conjugated Monoclonal CD45 Antibody-Based Nonmyeloablative Conditioning for Stem Cell Gene Therapy. Human gene therapy. 2015; 26(6):399-406. PubMed [journal] PMID: 25919226, PMCID: PMC4492596

Gori JL, Butler JM, Chan YY, Chandrasekaran D, Poulos MG, Ginsberg M, Nolan DJ, Elemento O, Wood BL, Adair JE, Rafii S, Kiem HP. Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells. The Journal of clinical investigation. 2015; 125(3):1243-54. PubMed [journal] PMID: 25664855, PMCID: PMC4362238

2014

Kiem HP, Arumugam PI, Burtner CR, Fox CF, Beard BC, Dexheimer P, Adair JE, Malik  P. Pigtailed macaques as a model to study long-term safety of lentivirus vector-mediated gene therapy for hemoglobinopathies. Molecular therapy. Methods & clinical development. 2014; 1:14055. PubMed [journal] PMID: 26052523, PMCID: PMC4448740

Wang CX, Sather BD, Wang X, Adair J, Khan I, Singh S, Lang S, Adams A, Curinga G, Kiem HP, Miao CH, Rawlings DJ, Torbett BE. Rapamycin relieves lentiviral vector transduction resistance in human and mouse hematopoietic stem cells. Blood. 2014; 124(6):913-23. PubMed [journal] PMID: 24914132, PMCID: PMC4126331

Burtner CR, Beard BC, Kennedy DR, Wohlfahrt ME, Adair JE, Trobridge GD, Scharenberg AM, Torgerson TR, Rawlings DJ, Felsburg PJ, Kiem HP. Intravenous injection of a foamy virus vector to correct canine SCID-X1. Blood. 2014; 123(23):3578-84. PubMed [journal] PMID: 24642749, PMCID: PMC4047497

2012

Gori JL, Chandrasekaran D, Kowalski JP, Adair JE, Beard BC, D'Souza SL, Kiem HP.  Efficient generation, purification, and expansion of CD34(+) hematopoietic progenitor cells from nonhuman primate-induced pluripotent stem cells. Blood. 2012; 120(13):e35-44. PubMed [journal] PMID: 22898598, PMCID: PMC3460690

2011

Watts KL, Adair J, Kiem HP. Hematopoietic stem cell expansion and gene therapy. Cytotherapy. 2011; 13(10):1164-71. NIHMSID: NIHMS349489 PubMed [journal] PMID: 21999373, PMCID: PMC3291512

Zhong B, Watts KL, Gori JL, Wohlfahrt ME, Enssle J, Adair JE, Kiem HP. Safeguarding nonhuman primate iPS cells with suicide genes. Molecular therapy : the journal of the American Society of Gene Therapy. 2011; 19(9):1667-75. PubMed  [journal] PMID: 21587213, PMCID: PMC3182363

Zhong B, Trobridge GD, Zhang X, Watts KL, Ramakrishnan A, Wohlfahrt M, Adair JE,  Kiem HP. Efficient generation of nonhuman primate induced pluripotent stem cells. Stem cells and development. 2011; 20(5):795-807. PubMed [journal] PMID: 21058905, PMCID: PMC3128793

2010

Matsuoka T, Adair JE, Lih FB, Hsi LC, Rubino M, Eling TE, Tomer KB, Yashiro M, Hirakawa K, Olden K, Roberts JD. Elevated dietary linoleic acid increases gastric carcinoma cell invasion and metastasis in mice. British journal of cancer. 2010;  103(8):1182-91. PubMed [journal] PMID: 20842125, PMCID: PMC2967057