• Projects
  • Lab Members
  • Genomics Data
    • Isw1 Transcript Array
    • Isw1-NuA4-Swr1 Transcript Arrays
    • Isw2 location analyses
    • isw2 nhp10 Replication and Expression Data
    • Dls1 Transcript Array
    • Histone H4 Basic Patch Mutant Transcript Array
    • Global Nucleosome Mapping
    • NFR Annotation and Cryptic RNA Analysis
    • Increase in chromatin accessibility (NCAM) at DNA replication sites
  • Protocols
  • Publications
  • Contact
  • Fredhutch.org
  • About Us
  • Ways To Give
Fred Hutch Logo

Tsukiyama Lab

Tsukiyama Lab

Fred Hutch Logo
  • Projects
  • Lab Members
  • Genomics Data
    • Isw1 Transcript Array
    • Isw1-NuA4-Swr1 Transcript Arrays
    • Isw2 location analyses
    • isw2 nhp10 Replication and Expression Data
    • Dls1 Transcript Array
    • Histone H4 Basic Patch Mutant Transcript Array
    • Global Nucleosome Mapping
    • NFR Annotation and Cryptic RNA Analysis
    • Increase in chromatin accessibility (NCAM) at DNA replication sites
  • Protocols
  • Publications
  • Contact
  • Tsukiyama Lab
  • Protocols

Protocols

Pipette wells

Antibody cross-linking

Chromatin Assembly Protocol using Drosophila S150 extract

Chromatin IP (ISW 1/2)

DNase I footprinting

Expression, Purification and Reconstitution of Recombinant Yeast Histones

FLAG purification

FLAG-tagging (3 copies) yeast proteins by PCR 

Nucleosome Spacing Assay

Nucleosome Mapping-Mnase

Quick Chromatin Digestions

TALO8 Purification

Fred Hutch Logo
Fundraise
Donate
Facebook Twitter YouTube Pinterest Instagram LinkedIn
Quick Links
  • Find a Job at Fred Hutch
  • Subscribe to Hutch News
Fred Hutchinson Cancer Center
Fred Hutchinson Cancer Center | 1100 Fairview Ave. N., Seattle, WA 98109
© 2025 Fred Hutchinson Cancer Center, a 501(c)(3) nonprofit organization.