Stoddard Lab

Stoddard Lab

Stoddard lab members

Connor O’Shaughnessy

Our Research

The overall goal of our laboratory is to characterize the structure/function relationships of a variety of enzymatic catalysts at the atomic level. Much of this work is being extended into efforts to engineer novel structures and properties onto existing protein and enzyme scaffolds. A unifying theme between many of the individual projects is the selection and engineering of these enzymes for targeted therapeutic and/or biotech/industrical applications.

The tools employed by our lab are X-ray crystallography, computer modeling, and genetic manipulation of the molecules of interest, combined with biochemical analyses of function.

Recent Publications

eLife 2019

Forsberg KJ, Bhatt IV, Schmidtke DT, Javanmardi K, Dillard KE, Stoddard BL, Finkelstein IJ, Kaiser BK, Malik HS (2019) “Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome” eLife Sep 10;8. pii: e46540. doi: 10.7554/eLife.46540. PMID 31502535.

Nature Structural and Molecular Biology 2020

Correnti, C.E., Hallinan, J. P., Doyle, L. A., Ruff, R. O, Jaeger-Ruckstuhl, C. A., Xu, Y., Shen, B. W., Qu, A., Polkinghorn, C., Friend, D. J., Bandaranayake, A.D., Riddell, S. R., Kaiser, B. K., Stoddard,B. L. and Bradley, P. (2020) "Engineering and functionalization of large circular tandem repeat protein nanoparticles" Nature Structural and Molecular Biology 27 (4): 342 – 350.  PMID 32203491

Structure 2020

 Lambert, A.R., Hallinan, J.P., Werther, R., Glow, D., and Stoddard, B.L. (2020) “Optimization of protein thermostability and exploitation of recognition behavior to engineer altered protein-DNA recognition” Structure. 2020 Jul 7;28(7):760-775.e8. doi: 10.1016/j.str.2020.04.009. Epub 2020 Apr 30. PMID 32359399