Journal Club

Crystals of a computationally designed protein
Crystals of a computationally designed protein Image by Lindsey Doyle

2021-2022 FHCRC Structural Biology and Molecular Biophysics Journal Club

Fred Hutch's Structural Biology and Molecular Biophysics Journal Club is held from 11:00 am - 12:00 pm on Thursdays.

Date Presenter Topic / Manuscript
September 29 Dr. Barry Stoddard

"Structural and mechanistic studies of a bifunctional homing endonuclease and intron maturation factor, I-AniI”

Suggested Reading: Liu, D. et al. (2022) “Sub-3-Å cryo-EM structure of RNA enabled by engineered homomeric self-assembly” Nature Methods 19: 576 - 585  (PubMed 35501384)

October 20 Dr. Melody Campbell Melody will present some of her research
November 10 Nick Hurbert (Pancera Group)

"The structure of human B cell receptors"

Suggested Readings:

Ma X, Zhu Y, Dong D, Chen Y, Wang S, Yang D, Ma Z, Zhang A, Zhang F, Guo C, Huang Z. Cryo-EM structures of two human B cell receptor isotypes. Science. 2022 Aug 19;377(6608):880-885. doi: 10.1126/science.abo3828. Epub 2022 Aug 18. PMID: 35981028.

Su Q, Chen M, Shi Y, Zhang X, Huang G, Huang B, Liu D, Liu Z, Shi Y. Cryo-EM structure of the human IgM B cell receptor. Science. 2022 Aug 19;377(6608):875-880. doi: 10.1126/science.abo3923. Epub 2022 Aug 18. PMID: 35981043.

December 1 Peter Rupert (Strong Group) "Mesothelin: antibody development structural studies"
December 15 Jeremy Hollis (Campbell Group)

"The Structural Basis of Gamete Interactions"

Suggested Reading:

Ohto U, Ishida H, Krayukhina E, Uchiyama S, Inoue N, Shimizu T. Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. Nature. 2016 Jun 23;534(7608):566-9. doi: 10.1038/nature18596. Epub 2016 Jun 15. PMID: 27309808.

Aydin H, Sultana A, Li S, Thavalingam A, Lee JE. Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex. Nature. 2016 Jun 23;534(7608):562-5. doi: 10.1038/nature18595. Epub 2016 Jun 15. PMID: 27309818; PMCID: PMC5319863.

January 5 Adam Nguyen (Campbell Group)

"Utilizing hydrogen-deuterium exchange coupled with mass spectrometry as a complementary tool for cryoEM and structural biology"

Suggested readings:

Fagnen et al. (2020) "New Structural insights into Kir channel gating from molecular simulations, HDX-MS and functional studies" Sci Reports 10: 8329.

Fernandes et al. (2022) "Cryo-electron microscopy unveils unique structural features of the human Kir2.1 channel" Science Advances 8 (38).

January 26 Ulysses Foote (Strong Group) R.J. Borges, F.F. Cardoso, C. de Carvalho et al., Structural and functional studies of a snake venom phospholipase A2-like protein complexed to an inhibitor from Tabernaemontana catharinensis, Biochimie,
February 16 Thamiya Vasanthakumar (Campbell Group)

Wang et al. (2023) “Structural basis of V-ATPase Vo region assembly by Vmal2p, 21p and 22p” Proc. Nat. Acad. Sci. (advance access)

March 9 Gargi Kher (Pancera Group) Vaney, MC., Dellarole, M., Duquerroy, S. et al. "Evolution and activation mechanism of the flavivirus class II membrane-fusion machinery." Nat Commun 13, 3718 (2022).   DOI
March 30 Madison Kennedy (Stoddard Group) Lurie C.L. et al. and Ohi, M.D. (2020) “Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex” eLife. DOI
April 20 Andres Fernandez (Campbell Group)  
May 11 Juliana Young (Stoddard / McGuire Groups)  
June 1 Matthew Chan (Campbell Group)  
June 22 Morgan McGovern (Pancera Group)