Data Collection FAQ

Diffraction pattern of RA-61
Diffraction pattern of RA-61 Image by Lindsey Doyle

Fall 2025

Please contact Lindsey Doyle (ldoyle@fredhutch.org) to be added to the e-mail list

  • BL 5.0.1    Wednesday, October 15      2pm-5am    
  • BL 5.0.2    Thursday, December 18      10am-5:30pm    

Two days before data collection (i.e. if data collection is on a Thursday then crystals will ship out on Tuesday). Crystals must be loaded into the shipping dewar by 2:30 pm.

Updated May 2022

All publications based on work done in whole, or in part, at the ALS/BCSB should include the following acknowledgment:

The Berkeley Center for Structural Biology is supported in part by the National Institutes of Health, National Institute of General Medical Sciences, and the Howard Hughes Medical Institute. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Pilatus detector was funded under NIH grant S10OD021832. The ALS-ENABLE beamlines are supported in part by the National Institutes of Health, National Institute of General Medical Sciences, grant P30 GM124169

S10 instrumentation grant: 1S10OD028581-01

  • X-ray Unit: Rigaku XtalLAB Synergy-R
  • Detector: Rigaku HyPix-6000HE hybride photon counting detector
  • Generator: PhotonJet-R
    • MicroMax-007HF microfocus rotating anode with a Copper source
    • Optics: VHF-style integrated
    • Pixel size: 100 μm
  • Software: CrysAlisPro

Sign up on the white boards in the X-ray suite (B3-170).

Hit the large, red EMERGENCY button on the generator’s front panel.

1.54Å

  • If there’s no shadow for the beam stop then...
    • the cover is still on the detector 
    • a water fault alarm has caused the X-rays to turn off (note in this case that the cryo turns off as well so remove your crystal).
  • If there’s a very faint shadow for the beam stop check to make sure X-rays are ramped up to 40 kV/30 mA.
  • If there’s a full shadow for the beam stop then your crystal didn’t diffract. Sorry.

All users should be removing their data files at the end of screening/collection. 

Check with other user to see if they can remove any files.

Let Lindsey know so she can schedule a purge.

Check with Lindsey: B3-175, x4066, ldoyle@fredhutch.org

Call AirGas at 1-800-224-7427, press 2. Our customer number is 2336490.

Turn off the Cryo System overnight.

That’s the cold-water alarm on the Haskris. Let Lindsey in B3-175 know (or Peter in B3-177 if Lindsey isn’t around).

Pull out the reset button and turn it clockwise. Turn the power on. Once the run lamp is on and the vacuum is stable, turn on the X-rays and dial them up.