The facility supporting structural biology at the Fred Hutchinson Cancer Research Center is housed in the the third floor of the Weintraub (Basic Sciences) Building, B-wing. View map of (and directions to and around) the Hutch campus.
The Stoddard Lab is located directly adjacent to the X-ray and crystallization facilities, in B3-175. The lab consists of 4 bays, 8 benches (approximately 1500 sq. ft), supporting a staff of approximately 8 full time research staff plus the P.I. All bench/desks are supported by Mac workstations connected to the Center ethernet spine.
The X-ray diffraction data collection suite, which supports three investigators, consists of a Rigaku Micromax-007HF rotating anode generator, one RAXIS-IV++ imaging plate detector, and one Saturn 994+ CCD area detector. Each is iluminated with X-rays focused with a Confocal Varimax Optics Systems, and each speciment is cooled by X-STREAM 2000 low temperature cryocooling system. Two computer workstations provide the necessary platforms for data collection and processing. Each workstation is directly linked to its own high-capacity hard-drive and backup device for direct , efficient data collection and archiving.
Two separate rooms house shelf-space and incubators (Sheldon Manufacturing, Inc.) for crystal growth at several ambient temperatures (12, 15, 18, 22, and 25 degrees C) and at 4 degrees C.
As well, the FHCRC is a member of the Principle Research Team (PRT) for beamlines 5.0.1 and 5.0.2 at the Advanced Light Source (ALS) Synchrotron Facility at the University of California, Berkeley (Lawrence Berkeley National Laboratory).
Here are photos and links to information for some of our major pieces of wet lab instrumentation, ranging from analysis of solution properties, to thermodynamic binding assays, to purification and crystallization workstations.