The facility supporting structural biology at the Fred Hutchinson Cancer Research Center is housed in the the third floor of the Weintraub (Basic Sciences) Building, B-wing. View map of (and directions to and around) the Hutch campus.
The Stoddard Lab is located directly adjacent to the X-ray and crystallization facilities, in B3-175. The lab consists of 4 bays, 8 benches (approximately 1500 sq. ft), supporting a staff of approximately 8 full time research staff plus the P.I. All bench/desks are supported by Mac workstations connected to the Center ethernet spine.
The X-ray diffraction data collection suite, which supports three investigators and their collaborators, consists of a Rigaku XtaLAB Synergy-R utilizing a PhotonJet-R Copper rotating anode generator with integrated VHF-style optics and divergence slit. Data is recorded in shutterless mode on a HyPix-6000HE hybrid photon counting detector with a distance range of 30-250 mm and telescoping 2Theta arm. Samples are kept cooled with an Oxford Cryosystems Cryonozzle and mounted on a universal goniometer, allowing samples to move at 10 degress per second and rotate around 3 axis with the detector rotation providing an additional axis. The XtalLAB Synergy is controlled at a computer workstation running CrysAlisPro. An additional computer workstation running HKL2000 for processing syncrotron data is available. A crystal cooling workbench is located directly adjacent to the XtalLAB Synergy unit with a microscope, cryo-tools, dewars and other tools necessary for successful flash-cooling of crystals..
Two separate rooms house shelf-space and incubators (Sheldon Manufacturing, Inc.) for crystal growth at several ambient temperatures (12, 15, 18, 22, and 25 degrees C) and at 4 degrees C.
As well, the FHCRC is a member of the Principle Research Team (PRT) for beamlines 5.0.1 and 5.0.2 at the Advanced Light Source (ALS) Synchrotron Facility at the University of California, Berkeley (Lawrence Berkeley National Laboratory).
Here are photos and links to information for some of our major pieces of wet lab instrumentation, ranging from analysis of solution properties, to thermodynamic binding assays, to purification and crystallization workstations.