Fred Hutch Electron Microscopy & CryoEM Core

Melody is the Scientific Director of the Electron Microscopy Core that is a part of Shared Resources at Fred Hutch.  Theo Humphreys joins us from PNCC in December 2022 to lead the EM Core as the Associate Director.

See the shared resource website.

Located in the Thomas building, this newly renovated facility houses cutting-edge instrumentation, including a 200 kV ThermoFisher Glacios X-FEG electron microscope equipped with a Gatan K3 detector and a 120 kV ThermoFisher Talos 120C LaB₆ microscope equipped with a Ceta camera. The Cryo-EM Core includes a centralized sample prep area and is set up for on-the-fly data processing and seamless data transfer to the Hutch HPC Cluster.

Additionally, scientists at the Hutch have access to a 300 kV Titan Krios equipped with a Gatan energy filter and K3 camera up the street at the Arnold and Mabel Beckman Center for Cryo-EM at the University of Washington, as well as instrumentation and resources at the Pacific Northwest Center for Cryo-EM (PNCC). 

Glacios + K3

So far the lab has determined structures of apoferritin at 2.4 Å and integrin at 3.1 Å using our Glacios + K3. For a timelapse of the set up (and acknowlegements) click here.

• High Tension: 200 kv
  
• Spherical Aberration (Cs): 2.7 mm
• Nominal Magnification @ 36,000x : 44,563x
• K3 detector pixel size: 5 µm, (1.122 Å/pixel @ 36,000x; 0.0561 Å/pixel super res)
• Dimensions: 5760 x 4092 physical pixel
• Typical Dose: 50 e-/Å2
• Typical Frames: 99
• Dose Rate: 8
• Exposure time: 7.9s

Talos L120C

The long duration dewar means you never have to fill the cold trap, supporting multiday data collections with no user intervention.

Sample Prep

We also have our own stain bench and glow discharger upstairs in the Campbell Lab.

Glacios Time-lapse

Time-lapse of the Glacios installation at Fred Hutch that happened from November 9, 2020 to March 17, 2021. THANK YOU so much to all who helped make this happen!

Melody, Theo, Anvesh, & Steve would like to thank everyone who gave their time and expertise so generously to get this microscope (and the entire cryoEM suite and new Shared Resource) up and running at the Hutch.