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Fred Hutch CryoEM Core

Melody is the Scientific Director & Caleigh is the Facility Manager of the Cryo-EM Core that is a part of Shared Resources at Fred Hutch.

See the shared resource website here.

Located in the Thomas building, this newly rennovated facility houses cutting-edge instrumentation including a 200 kV ThermoFisher Glacios X-FEG electron microscope equipped with a Gatan K3 detector and a 120 kV ThermoFisher Talos 120C LaB₆ microscope equipped with a Ceta camera. The Cryo-EM Core includes a centralized sample prep area and is set up for on-the-fly data processing and seamless data transfer to the Hutch HPC Cluster.

Additionally, scientists at the Hutch have access to a 300 kV Titan Krios equipped with a Gatan energy filter and K3 camera up the street at the Arnold and Mabel Beckman Center for Cryo-EM at the University of Washington, as well as instrumentation and resources at the Pacific Northwest Center for Cryo-EM (PNCC).

Fred Hutch : Glacios + K3 | Talos | Sample Prep | Time-lapse

Glacios + K3

So far the lab has determined structures of apoferritin at 2.4 Å and integrin at 3.1 Å using our Glacios + K3. For a timelapse of the set up (and acknowlegements) click here.

Server rack for data storage & on-the-fly processing

Talos L120C

The long duration dewar means you never have to fill the cold trap, supporting multiday data collections with no user intervention.

Sample Prep

A couple additions coming soon: a Pelco easiGlow glow discharger and a Cressington carbon evaporator for Shared Resources. (We have our own stain bench and glow discharger upstairs as well.)

Glacios Time-lapse

Time-lapse of the Glacios installation at Fred Hutch that happened from November 9, 2020 to March 17, 2021. THANK YOU so much to all who helped make this happen!

Melody & Caleigh would like to thank everyone who gave their time and expertise so generously to get this microscope (and the entire cryoEM suite and new Shared Resource) up and running at the Hutch.

The new microscope suite was made possible with unmeasurable internal help and support from:

Fred Hutch presidents Garry Gilliland & Tom Lynch for their initial & continued support.

Hutch Basic Sciences including:

Sue Biggins
Barry Stoddard
Christy Majorowicz
Luna Yu

Hutch Engineering and Facilities including:

Al Spencer
Steve Matthes
Mark Hungerford
Michael Carney
& many, many, others

Hutch Center IT and HPC including:

Loren Greenen
Ben McGough
Chris Rogers
Irina Kalmus
Russell Ison
Jim Rudd
Tom Grandey
Tyler Barwick
Dirk Petersen

Hutch Shared Resources including:

Jeff Delrow
Peng Guo
Steve McFarlane
Bobbie Schneider
Stuart Tenney
Marion Dorer
Liz Hirunmetakij
Landon Howell

Hutch Purchasing Shipping & Receiving including:

Reed Lengyel-Vaught
Jenny Adams
Reggie Moyer

CryoEM SR Website Team & Media including:

Sara Norman
Jeremy Fisher
Jim Woolace
Regina Sologub
Susan Keown
Sabrina Richards
Robert Hood

Basic Sci Beta Testers:

Rachel Werther (Campbell Lab)
Jeremy Hollis (Campbell/Malik Labs)
Adam Nguyen (Campbell Lab)
Betty Shen (Stoddard Lab)

& external support from:

Hardware, Software, & Computing Advice

JC Ducom (Scripps)
Matt Harrison (UCSF)
Daniel Asarnow (UCSF)
Paul Thomas (UCSF)
David Veesler (UW)
Justin Kollman (UW)
Joel Quispe (UW)
Sasha Dickenson (UT Southwestern)
Michael Cianfrocco (UMich)

Scientific Hardware Installation & Support

ThermoFisher/FEI

Khang Lam
Mar Bulda
Jonathan Herrmann
Peter Stokes
Rex Hill
Kyle Hodgeson
Justin Blogg
Ty Campsey
John Grimes
Preston Evenson

Gatan

Jim Cook
Austin Green
Phil Manacop

Vibration Engineering Consulting

Joel Paul

Design & Construction Teams

SABArchitects

Biko Tabor

Lease Crutcher Lewis

Jacki Dunlap
Shane Weller
Austin Newton

Prime Electric

Amy Thomas
Yale Lance
Loren Wood

University Mechanical

Jag Singh
Ray Portello

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