Melody is the Scientific Director & Caleigh is the Facility Manager of the Cryo-EM Core that is a part of Shared Resources at Fred Hutch.
See the shared resource website here.
Located in the Thomas building, this newly rennovated facility houses cutting-edge instrumentation including a 200 kV ThermoFisher Glacios X-FEG electron microscope equipped with a Gatan K3 detector and a 120 kV ThermoFisher Talos 120C LaB6 microscope equipped with a Ceta camera. The Cryo-EM Core includes a centralized sample prep area and is set up for on-the-fly data processing and seamless data transfer to the Hutch HPC Cluster.
Additionally, scientists at the Hutch have access to a 300 kV Titan Krios equipped with a Gatan energy filter and K3 camera up the street at the Arnold and Mabel Beckman Center for Cryo-EM at the University of Washington, as well as instrumentation and resources at the Pacific Northwest Center for Cryo-EM (PNCC).
So far the lab has determined structures of apoferritin at 2.4 Å and integrin at 3.1 Å using our Glacios + K3. For a timelapse of the set up (and acknowlegements) click here.
The long duration dewar means you never have to fill the cold trap, supporting multiday data collections with no user intervention.
A couple additions coming soon: a Pelco easiGlow glow discharger and a Cressington carbon evaporator for Shared Resources. (We have our own stain bench and glow discharger upstairs as well.)
Time-lapse of the Glacios installation at Fred Hutch that happened from November 9, 2020 to March 17, 2021. THANK YOU so much to all who helped make this happen!
Melody & Caleigh would like to thank everyone who gave their time and expertise so generously to get this microscope (and the entire cryoEM suite and new Shared Resource) up and running at the Hutch.
Hutch Basic Sciences including:
Hutch Engineering and Facilities including:
Hutch Center IT and HPC including:
Hutch Shared Resources including:
Hutch Purchasing Shipping & Receiving including:
CryoEM SR Website Team & Media including:
Basic Sci Beta Testers:
& external support from:
Hardware, Software, & Computing Advice
Scientific Hardware Installation & Support
Vibration Engineering Consulting
Design & Construction Teams
Lease Crutcher Lewis