News


2020 | 2021 | 2022

September 2022

01: NOA for the lab's first NIH R35 MIRA! 🎉

August 2022

26: Passed with flying colors and to rave reviews! Congrats to Jeremy for (very) successfully passing his qualifying exam! A first for the Campbell lab, and the first of the whole 2020 MCB cohort! Amazing written proposal, presentation, and the questions were fielded like a pro. More than excited about the projects as they continue to move forward. (Also, thank you to Thamiya for this amazing three-layer cinnamon toast crunch integrin cake!)

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cake

25: Andres was awarded a T32 fellowship from the Molecular Biophysics Training Program!  He wrote an extraordinarily clear and well-thought-out proposal that he presented expertly! Congrats, Andres (celebration coming soon)!

andres

22: Farewell to Caleigh! Grateful for the ~2 years we had together and all of the fantastic work she has done to establish the EM Center. Best of luck, Caleigh, & knock em dead at Genetech!

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For a hot second the lab was 10 people!

12: When Kevin sets their mind to something, Kevin makes it happen! Kevin presented their research at the Summer Undergraduate Research Program (SURP) final poster session. Not only did they put together an aesthetically beautiful poster, but they have made significant progress on their project, and presented it with such knowledge and enthusiasm that they won the 'Best Presentation award! You nailed it Kevin-- the whole lab is proud!

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"Here's me winning"

12: Early birds get the worm! Can't believe we've filled all of the desks in the lab already, but this overlap will only be for a couple of weeks before Kevin heads back to Western Washington. Thanks to Matthew for being so patient with the temp desk.

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10: Chewbacca, our HPLC finally has all of the pieces and is up and running!

08: Matthew Starts! Matthew joins us from Shulka group at the University of Illinois Urbana-Champaign where he worked to uncover the molecular mechanisms of neurotransmitter transport in neurons!  Matthew is part of the Campbell lab and Rasi lab. Before even starting, Matthew designed, wrote, and was awarded the prestigious Mahan fellowship. We've also learned he has quite an artistic streak and we're thrilled he has joined the group Welcome Matthew, congrats & we're excited that you're here!

01: Melody receives the George Palade award from the Microscopy Society of America.

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July 2022

30: Melody gives a plenary talk at the student-run PMCx60 at the Microscopy and Microanalysis meeting in Portland! 

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20: Not to show off, but definitely to show off, look at these unbelievable flowers that the ever-talented Jeremy folded, assembled, and glitter sprayed from of an old poster....

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June 2022

30: The lab turns TWO! Paddleboarding and Pizza to celebrate.

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20: No surprises here, but congrats to Thamiya who nailed her invited talk, "Overcoming Instability and Heterogeneity for Cryo-EM of the V1 Complex" at the 3D Electron Microscopy GRC. If anyone needs a speaker to talk about cutting-edge technical advances in cryoEM, you know where to find her!

17: Wasn't quite sure what to expect but Melody had an absolute blast presenting the research we do as part of the Bandit Theater's Mad Science Comedy Show. Thanks to all who came out to help support me embarrass myself to a sold out audience, thanks to Erik for nominating me for this opportunity, but most of all thanks to the wonderful comedians who riffed on integrins & immunity. Very proud that after less than two years we already have a(n improv) musical about the lab 🎶

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Photo by Paula Yahn
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bandit

14: Melody is selected as a Pew Biomedical Scholar! Read more about this award and our plans here.

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Ye Olde Glow Discharger Photo strikes again...

Photo by Robert Hood

13: Welcome to Kevin Alexander Estrada Alamo who visits us for the summer from the Amacher lab at Western Washington University where they have been using x-ray crystallography to study the selectivity-determining loops in SH2 domains. Kevin is keen to learn cryoEM and will be working with Jeremy & Rachel for the next two months. Welcome to Kevin, we can't wait to get started!

13: Thank you to Matthew Ross, the truly talented science writer who somehow knitted my meandering stream of consciousness answers to his interview questions into this eloquent, cohesive, and overtly-flattering spotlight. (More than a few "ums" and "likes" may have been edited out of the direct quotes...)

7: Could barely sneak a photo since his poster was so crowded for both sessions, but Andres did a fantastic job presenting his rotation project at the MCB poster session.

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Andres presenting

3: Second annual 3rd Floor NE tri-lab picnic. Couldn't have wished for better weather.*
*Given the initial forecast and all previous rainfall/temperature data points this year 😭

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bbq
fun
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whole group
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Credit to Erik Black (and assistant) for all Campbell lab group photos

May 2022

27: Andres joins the lab so we're celebrating with Man vs Fries on the rooftop. Welcome to the lab Andres-- excited for what you and your project will bring!

andres vs fries

Andres vs. Fries: Andres won.

20: It must be presentation season! Jeremy presented his poster at the Molecular Biophysics Training Grant Program (MBTG) retreat & Adam gave the first Campbell Lab Friday Night Talk.

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Jeremy presenting his poster

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Adam giving his talk

17: Thamiya did an outstanding job presenting a technical talk on how she overcame dissociated complexes in ice at the Seattle EM Super Group Meeting

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Thamiya giving her talk

6: New People = New DATA 🤩🤩🤩

gfp ice

Thamiya and Andres have hit the ground running

April 2022

29: Pre-gaming next week's Beer Hour with a Bai-Singhvi-Campbell pre-Beer Hour (& pretending that it is warmer out than it actually is)

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Pis
All Photos by Luna Yu

18: Jeremy has truly finessed how to be dual-advised: twice the advice, twice the critiques, twice the meetings, twice the celebrations...

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All Photos by Luna Yu

15: Thamiya arrives! Thamiya was the SECOND person who signed on to be in the lab back in December of 2020! Her grad project grew some new interesting directions so her timeline was shifted, but we're thrilled that she is finally here.

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& Jeremy made coffee-cake cup-cakes to celebrate

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4: Jeremy wins an NSF-GRFP (National Science Foundation Graduate Research Fellowships Program)! Co-advised with Harmit Malik, Jeremy initiated the collaboration between our labs, devised and designed this innovative project completely on his own, and is diligently carrying it out. Couldn't be more deserving of this recognition and forever thrilled that he's a part of the lab.

jeremy glacios

1:  Stain Aliquoting Party & Peak Magnolia Season

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March 2022

28: Andres Fernandez starts his rotation (at the yellow bench). Welcome, Andres! We've got big plans...

yellow bench

23: Special edition journal club today where we discussed incoming post-doc Thamiya's most recent manuscript in bioRxiv, immediately followed by virtually attending her thesis defense at Sick Kids in Toronto! A beautiful and artistic presentation clearly outlining an impressive body of work. Congratulations to Dr. Vasanthakumar!

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22: Our Hutch @ Home Tour is live! Organized by Jeanne and the Team at SciEdHutch, filmed by Caren, and starring the whole lab! Proud of how clearly and enthusiastically everyone in the lab explained what we do.

rachel explains BLI

Rachel explaining the BLI!

21: Ongoing grad student results... 🤩

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time to freeze grids

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nyquist here we come...

18: We are celebrating: the fact we're allowed to eat together on campus (pizza) Caleigh's birthday (apple tarte), and [EMBARGOED] (champagne)! More soon...

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Happy Birthday, Caleigh!!

18: In preparation for a post-doc and a rotation student starting in April we had to re-organize the lab to make space. Can't believe how quickly we've grown (and how much equipment we have).


February 2022

28: Is there such thing as too many birthday treats?

treats

The answer is no.

07: It's been almost exactly two years since I gave my last in-person seminar (as a job interview)! Thrilled to get to give a seminar in person again today to the undergrads at Seattle Pacific University. Very impressed by the questions and enthusiasm and 🤞🏼🤞🏼🤞🏼 that maybe I've converted a couple of future structural biologists. Thanks again to John Douglass for the invite & tour of the science building.

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January 2022

20: Grad school interview season is upon us! Proud of both Jeremy and Adam for taking on leadership roles and extra responsibilities for MCB and BPSD recruitment, respectively.

03: Sweets, arts & crafts, and new polymers: no better way to kick off the new year! Many thanks to our across-the-sea friends in the Autzen lab!

glaedig

December 2021

23: Warmest* Wishes! Grateful for how far we've come this year.

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*Structure was frozen in vitreous ice and imaged at -170 ˚C. "Warmest" only applies to a subset of wishes.

21: Happy Solstice! Miracle to end all miracles, sonata, our main 10 GPU / 104 CPU server, has arrived. No excuse now not to try absolutely everything while processing data (and there are so many things to try)!

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17: I forgot to take photos most of the night, but the lab holiday party was a success! Food, wine, white elephant, and of course... arts & crafts.

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16: We've been waiting for over three months for our GPU server to come, but in the meantime, inspired to build this gingerbread server for the compbio gingerbread contest. Fun fact: licorice cables are more difficult to manage than real cables...

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13: The Computer Lab Cake Room Christmas Room is sufficiently cozy now that we're heading into that time of year when we get less than 8.5 hrs of sunlight per day. 😭

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13: Grateful for the support from Obliteride and these extremely kind handwritten thank you notes from Obliteride supporters.

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08: Won the door decorating contest-- the lab is on a roll! (Helps to have a professional set designer on the team.) Thrilled to be at a place that is working so hard to constantly organize these ray-of-sunshine events even when the pandemic is dragging us down.

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Final Design

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Initial Vision

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Work in progress

02: Last birthday of the season seamlessly transitioning into the holiday season! Happy Birthday, Jeremy!

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"Dreaming of Mille-Feuille"
Jeremy Hollis, 2021
Digital Photography
2048 x 1536


November 2021

24: Happy Labsgiving! Grateful for many things this year including these grad students who organized a surprise sushi lunch 🍣😊

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19: Really proud of the whole lab, especially Jeremy and Adam for making it though grant writing and fellowship writing season. As a lab, we've submitted THIRTEEN proposals since the summer. Received two so far, 🤞🏼🤞🏻🤞🏼🤞🏾🤞🏻for more.

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09: Big moves in the Campbell Lab!

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L O L just kidding the fridge is broken

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04: Progress...

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October 2021

29: Absolute blast this year celebrating Halloween and carving pumpkins. Winning the "most artistic" category was just the icing on the cake.

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pumpkin carvers 2021
halloween zoom 2021
pumpkin submission 2021
mr mummy

26: 🙌🏼

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25: Picked up our pumpkins today: big plans this year for the Basic Sciences /Human Bio pumpkin carving contest! Thanks to Caleigh for hosting the our first annual pumpking carving. 🎃🎃🎃🎃

jeremy pumpkins 2021
pumpkins getting started 2021
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Pumpkin carving begins on the orange bench

Pumpkin carving begins on the orange bench

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25: Next birthday, next cake for Adam 🎂🎂🎂

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19: Campbell lab birthday season beings: First up, Christy & Rachel!

rachel bday 2021

15: Over ten years in a box and the next gen Group Meeting Gopher (G.M. Gopher) starts a new life as 'a reminder for who is presenting next week.' Chuffed to carry on this tradition from my time in the Karbstein Lab.

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How it's going (2021)

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How it started (2011)

01: Thanks to a generous donation from the Koss Family, we'll be able to pursue an antibody discovery project using the Antibody Technology Shared Resource at Fred Hutch! We're hoping these experiments will help us better understand the mechanism of integrin activation. Proud of this first grant awarded to the lab!


September 2021

18: Three talks in three days! Different audiences, different lengths, different content.... Probably won't agree to that time line again, but this last one was truly an honor to take part in! Read more about this outstanding partnership here.

SEP2021

17: Campbell Lab's on a ROLL. Even though he was one of the most junior scientists of the 38 participating (rising 2nd year grad student), the extraordinary Adam won first price at the Fred Hutch 40th Anniversary Basic Sciences Retreat Poster Session!!

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Balloons to celebrate Adam's big win!

Luna Yu

14: Lab sushi lunch to celebrate the fantastic Jeremy and his well earned T32 Molecular Biophysics Training Program Grant! 🎉

sushi selfie

You can't tell from the photo but Jeremy and Adam are definitely wearing matching hoodies.


August 2021

16: Second lab flood (on a much bigger scale than our leaky fridge)!!  🙌 to Singhvi & Bai labs for helping us panic-mop and 🙌🙌🙌to Hutch facilities for cleaning everything up so quickly. Fortunately, we had moved our workstations, etude and scherzo, the previous week.

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July 2021

14: Celebrating our first labbiversary with paddle boarding & pizza.

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11: Finally finished the Glacios time-lapse!

07: Super excited to host two fantastic seminar speakers this week! Cristina Puchades from UCSF shared her recent innovations, tips, & tricks to solve high resolution structures of membrane proteins, and Daniel Asarnow presented some of the intricate and comprehsive work he has done to characterize a collection of SARS-CoV-2 antibodies structurally and beyond.

First lab pizza party to celebrate two great talks, two submitted fellowships, AND the approved funding for a new carbon evaporator.

cristina daniel

06: Two graduate student fellowship proposals submitted and in the books! Couldn't be more proud of Adam & Jeremy for their innovate ideas and getting everything together so quickly after joining to get these shipped.


June 2021

25: Blast from the past when we did some cleaning up: personalized and monogrammed glass pipettes from when Hal Weintraub himself had his lab in this space over 25 years ago.

hal pipettes

10: Both Adam and Jeremy have have joined the lab!

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Nailed the jump on the first try-- still shocked

4: First Annual 3rd Floor NE Picnic at Golden Gardens with the Bai & Singhvi Labs was a blast!

bai singhvi campbell lab bbq 2021

4: First Second Lab Selfie

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2: Most entertaining instrument training yet on the BLI. Picked up some fantastic tips for setup and the new software & ended with a group rally to get Adam some last minute extra data for his rotation talk next week.

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May 2021

19: Celebrating first data collection using Leginon on the Talos. Absolutly heroic efforts on the part of Caleigh and the SciComp Team (esp. Ben, Chris, & Russell) to make this happen.

leginon talos

(& also celebrating once again the fact Jeremy makes the best cakes)

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Three-Layer Fruity Pebble Cake by Jeremy

12: Will it jinx is if I report that our TC room is really truly completely finished?

tissue culture room 1
tissue culture room 2
tissue culture room 3

11: Grad students are gettin it

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April 2021

24: Melody, Caleigh, and Barry Stoddard talk to GeekWire about the new cryoEM facility at the Hutch!

geekwire cryoEM campbell
Melody poses with an empty slide & her least favorite piece of equipment

23: Really grateful to have inherited Lilo and Stitch-- two water jacketed CO2 incubators-- from the Priess lab. They're pulling a lot of weight as we wait for our big new CO2 incubator to be fixed.

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... and they're lookin' cute doin' it

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19: Celebrating the fact we're all at least partially vaccinated, a full week of gorgeous weather, and the fact Jeremy makes the best cakes.

8: Glacios / K3 update: 2.4Å and aromatic rings!

2p4 helix plus

6: Campbell Lab in Bloom

magnolia 2021

2: One week into the rotation, and not to brag but check out how good both the grad students are at handling grids, making grids, & staining grids.

first negative stain
"This is how we used to collect data in the olden days before automation" I explain as I bumble though the low-dose kit on the Talos while we wait for the extremely delayed Leginon server hardware to come in...

March 2021

30: 😎 [Click for PUNS]

integrin smiles
Jeremy's Window Art
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Adam's Biosafety Cabinet Art

29: Welcome to TWO new rotation students: Jeremy Hollis  who joins us from the Seattle MCB program and Adam Nguyen  from the UW BPSD program. We're so excited to have you both here!

 

26: Prepping for *two* rotation students to start next week... 😱

Edit: By popular demand, product list can now be found here

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25: For a quick introduction for what we do in a cryoEM lab, read what Melody had to say in this interactive partner project between NIGMS and Scholastic: Imaging Lab.

24: Melody talks with Tom Lynch at "Science Says: Tech, terabytes, and transformation." They chat about cryoEM: what it is and what it can do at (and why it could have been advantageous to build the EM Center out at Snoqualmie Pass)

Science Says: Tech, terabytes, and transformation

Not up for the full hour of Melody making the extremely talented ASL interpreter's life difficult with phrases like 'high energy electrons,' 'vitreous ice,' and 'electromagnetic interference'? Check out this science snack instead...

...the SCIENCE is the snack.

19: Just half a day after sign-off, a ~2 hr inagural data collection by Caleigh of an apoferritin sample expressed and purified in the lab, and we have a 2.76Å map using 65 micrographs and ~27k particles.

apoF 2p76

18: Signed off on the Talos and Glacios electron microscopes today!

4: Welcome to our new "lab member" Scherzo. Ready to process data just as soon as the scopes are complete!

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how it's going
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(how it started)

2: Our BLI was installed today-- time to start measuring some binding affinities!

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February 2021

28: No news is good news?


January 2021

26: Signed off on the Gatan K3 detector for the electron microscope today.

15: It may not be the prettiest first western blot but it's *our* first western blot.

dino western blot

05: New year, new experiments!

first experiment

When growing mammlian cells keeping an ideal environment is extremely important for them to survive. We keep these cells on a moving shaker to help them stay aerated, and the shaker is inside of an incubator that's like south Florida in July: 37 ˚C and >80% humdity.


December 2020

22: Warmest* Wishes from the Campbell Lab! 2020 has been a wild time to open a lab, but with support from our neighbors at the Hutch I'm thrilled at what our little lab has set up so far. Excited for what's to come in 2021 with a post-doc joining and grad student rotating in the new year.

Warmest wishes from the campbell lab 2020

These little snowpeople are at nearly ≥98% viability & almost ready for transfection

17: The glow discharger is up!

glow discharger

glow dischargers gonna glow: A current of of 15 mA is passed though the chamber at low pressure which transfers energy to the electrons in gas molecules in the air. There is enough energy to generate the purple luminosity and ions are deposited on to anything within the chamber (in our case a EM grid). This makes the surface of the grid hydrophilic (negatively charged) and causes aqueous solutions to spread across the surface. This spreading helps us attain well dispersed particles for negative-stain imaging and thin even ice for cryo imaging.

15: Our TC setup is up and running and we're growing cells.

expi293 december 2020

Our Expi293 cells alive (mostly) & dividing.

11: The FEG has successfully been installed on the Glacios and we have a beam (albeit a very shakey and unstable one).

feg installed

& we didn't drop the FEG!

11: Our Shimadzu HPLC "Chewbacca" was set up today and we're ready* to run some FSEC. Chewbacca joins his new brother-- also called Chewbacca-- as the third HPLC-for-FSEC (to our knowledge) with this name.

han solos friend

*still need GFP-tagged protein

03: The Glacios is almost ready for the field emission gun (FEG)! The FEG is the electron source for our microscope and has a maximum accelerating voltage of 200,000 eV. It provides the small coherent beam of electrons that we use to image our samples at high resolutions.

feg prep

But what are the cupcake pans for?


November 2020

20: The Glacios microscope is plugged in, powered on, and moving!

The stage is moving, not the whole microscope. That would be bad. Video by Caleigh Azumaya

18: First Campbell Lab protein purified:

its just a protease to cleave tags

Maybe just a small victory for now, but this it felt like a huge relief because it means that we've sucessfully set up cell growth & protein expression and that the cells, media, buffers, incubators, FPLC, columns, and gels are all working as expected.

first gel
Images (& protein purification) by Rachel Werther

13: The lab is looking more like a lab!

how its going

This, of course, is Rachel's bench. Mine is not as tidy...

12: Glacios progress...

do we cut the green or red wires

A+ cable management

09: The two new electron microscopes-- a Talos L120C G2 and a Glacios Cryo-TEM-- also arrive at the Hutch today!

09: Welcome to Caleigh Azumaya who joins the Hutch as the facility manager for the CryoEM Center

melody the glacios and caleigh delivery

Left to Right: Glacios Box #1, Melody, Glacios Box #2, Caleigh, Glacios Box #3. The box between Melody and Caleigh weighs 925 kg (2040 lbs/ 1 rhinoceros) and pictured are only 3 of the 22 boxes delivered for the two microscopes.

Photo by Rachel Werther

05: Favorite lab accidental order so far: these comically small 2 mL scoops that I had assumed were much larger and ordered for scooping LB...

2mL scoops

Does this really count as news?


October 2020

28: First Campbell Lab pumpkin contest entry:

zoom pumpkin

15: Our FPLC, "BB8" is up and running! Nomenclature (BB8) follows standard chromatography naming conventions established while in the Cheng Lab.

BB8 Akta

Now just waiting for the column and injection kit to come in....

05: Welcome to our new technician, Rachel Werther, who joins us from Barry Stoddard's lab.


September 2020

25: Everyone likes a good before/after: Still some work to do, but movement towards finishing the TC room.

pre 2
before 2
after2

#glowup

24: Big equipment delivery today.

akta pure

2: Melody speaks with Tom Lynch at the Fred Hutch Virtual Town Hall about plans for the new CryoEM Center @ Fred hutch. Read the Full transcript here.


August 2020

10: Big changes coming to the Hutch EM Center! Today we officially broke ground and rennovations have begun for our new state-of-the-art cryoEM facility!

construction 01
construction 02

July 2020

01: Campbell Lab opens & we're hiring at all levels!

empty campbell lab photo july 2020

Ten empty lab benches waiting to be filled!