01: NOA for the lab's first NIH R35 MIRA! 🎉
26: Passed with flying colors and to rave reviews! Congrats to Jeremy for (very) successfully passing his qualifying exam! A first for the Campbell lab, and the first of the whole 2020 MCB cohort! Amazing written proposal, presentation, and the questions were fielded like a pro. More than excited about the projects as they continue to move forward. (Also, thank you to Thamiya for this amazing three-layer cinnamon toast crunch integrin cake!)
25: Andres was awarded a T32 fellowship from the Molecular Biophysics Training Program! He wrote an extraordinarily clear and well-thought-out proposal that he presented expertly! Congrats, Andres (celebration coming soon)!
22: Farewell to Caleigh! Grateful for the ~2 years we had together and all of the fantastic work she has done to establish the EM Center. Best of luck, Caleigh, & knock em dead at Genetech!
12: When Kevin sets their mind to something, Kevin makes it happen! Kevin presented their research at the Summer Undergraduate Research Program (SURP) final poster session. Not only did they put together an aesthetically beautiful poster, but they have made significant progress on their project, and presented it with such knowledge and enthusiasm that they won the 'Best Presentation award! You nailed it Kevin-- the whole lab is proud!
12: Early birds get the worm! Can't believe we've filled all of the desks in the lab already, but this overlap will only be for a couple of weeks before Kevin heads back to Western Washington. Thanks to Matthew for being so patient with the temp desk.
10: Chewbacca, our HPLC finally has all of the pieces and is up and running!
08: Matthew Starts! Matthew joins us from Shulka group at the University of Illinois Urbana-Champaign where he worked to uncover the molecular mechanisms of neurotransmitter transport in neurons! Matthew is part of the Campbell lab and Rasi lab. Before even starting, Matthew designed, wrote, and was awarded the prestigious Mahan fellowship. We've also learned he has quite an artistic streak and we're thrilled he has joined the group Welcome Matthew, congrats & we're excited that you're here!
20: Not to show off, but definitely to show off, look at these unbelievable flowers that the ever-talented Jeremy folded, assembled, and glitter sprayed from of an old poster....
30: The lab turns TWO! Paddleboarding and Pizza to celebrate.
17: Wasn't quite sure what to expect but Melody had an absolute blast presenting the research we do as part of the Bandit Theater's Mad Science Comedy Show. Thanks to all who came out to help support me embarrass myself to a sold out audience, thanks to Erik for nominating me for this opportunity, but most of all thanks to the wonderful comedians who riffed on integrins & immunity. Very proud that after less than two years we already have a(n improv) musical about the lab 🎶
13: Welcome to Kevin Alexander Estrada Alamo who visits us for the summer from the Amacher lab at Western Washington University where they have been using x-ray crystallography to study the selectivity-determining loops in SH2 domains. Kevin is keen to learn cryoEM and will be working with Jeremy & Rachel for the next two months. Welcome to Kevin, we can't wait to get started!
13: Thank you to Matthew Ross, the truly talented science writer who somehow knitted my meandering stream of consciousness answers to his interview questions into this eloquent, cohesive, and overtly-flattering spotlight. (More than a few "ums" and "likes" may have been edited out of the direct quotes...)
7: Could barely sneak a photo since his poster was so crowded for both sessions, but Andres did a fantastic job presenting his rotation project at the MCB poster session.
3: Second annual 3rd Floor NE tri-lab picnic. Couldn't have wished for better weather.*
*Given the initial forecast and all previous rainfall/temperature data points this year 😭
27: Andres joins the lab so we're celebrating with Man vs Fries on the rooftop. Welcome to the lab Andres-- excited for what you and your project will bring!
20: It must be presentation season! Jeremy presented his poster at the Molecular Biophysics Training Grant Program (MBTG) retreat & Adam gave the first Campbell Lab Friday Night Talk.
17: Thamiya did an outstanding job presenting a technical talk on how she overcame dissociated complexes in ice at the Seattle EM Super Group Meeting
6: New People = New DATA 🤩🤩🤩
29: Pre-gaming next week's Beer Hour with a Bai-Singhvi-Campbell pre-Beer Hour (& pretending that it is warmer out than it actually is)
18: Jeremy has truly finessed how to be dual-advised: twice the advice, twice the critiques, twice the meetings, twice the celebrations...
15: Thamiya arrives! Thamiya was the SECOND person who signed on to be in the lab back in December of 2020! Her grad project grew some new interesting directions so her timeline was shifted, but we're thrilled that she is finally here.
4: Jeremy wins an NSF-GRFP (National Science Foundation Graduate Research Fellowships Program)! Co-advised with Harmit Malik, Jeremy initiated the collaboration between our labs, devised and designed this innovative project completely on his own, and is diligently carrying it out. Couldn't be more deserving of this recognition and forever thrilled that he's a part of the lab.
1: Stain Aliquoting Party & Peak Magnolia Season
28: Andres Fernandez starts his rotation (at the yellow bench). Welcome, Andres! We've got big plans...
23: Special edition journal club today where we discussed incoming post-doc Thamiya's most recent manuscript in bioRxiv, immediately followed by virtually attending her thesis defense at Sick Kids in Toronto! A beautiful and artistic presentation clearly outlining an impressive body of work. Congratulations to Dr. Vasanthakumar!
22: Our Hutch @ Home Tour is live! Organized by Jeanne and the Team at SciEdHutch, filmed by Caren, and starring the whole lab! Proud of how clearly and enthusiastically everyone in the lab explained what we do.
21: Ongoing grad student results... 🤩
18: We are celebrating: the fact we're allowed to eat together on campus (pizza) Caleigh's birthday (apple tarte), and [EMBARGOED] (champagne)! More soon...
18: In preparation for a post-doc and a rotation student starting in April we had to re-organize the lab to make space. Can't believe how quickly we've grown (and how much equipment we have).
28: Is there such thing as too many birthday treats?
07: It's been almost exactly two years since I gave my last in-person seminar (as a job interview)! Thrilled to get to give a seminar in person again today to the undergrads at Seattle Pacific University. Very impressed by the questions and enthusiasm and 🤞🏼🤞🏼🤞🏼 that maybe I've converted a couple of future structural biologists. Thanks again to John Douglass for the invite & tour of the science building.
03: Sweets, arts & crafts, and new polymers: no better way to kick off the new year! Many thanks to our across-the-sea friends in the Autzen lab!
23: Warmest* Wishes! Grateful for how far we've come this year.
21: Happy Solstice! Miracle to end all miracles, sonata, our main 10 GPU / 104 CPU server, has arrived. No excuse now not to try absolutely everything while processing data (and there are so many things to try)!
17: I forgot to take photos most of the night, but the lab holiday party was a success! Food, wine, white elephant, and of course... arts & crafts.
16: We've been waiting for over three months for our GPU server to come, but in the meantime, inspired to build this gingerbread server for the compbio gingerbread contest. Fun fact: licorice cables are more difficult to manage than real cables...
Computer Lab Cake Room Christmas Room is sufficiently cozy now that we're heading into that time of year when we get less than 8.5 hrs of sunlight per day. 😭
13: Grateful for the support from Obliteride and these extremely kind handwritten thank you notes from Obliteride supporters.
08: Won the door decorating contest-- the lab is on a roll! (Helps to have a professional set designer on the team.) Thrilled to be at a place that is working so hard to constantly organize these ray-of-sunshine events even when the pandemic is dragging us down.
02: Last birthday of the season seamlessly transitioning into the holiday season! Happy Birthday, Jeremy!
"Dreaming of Mille-Feuille"
Jeremy Hollis, 2021
2048 x 1536
24: Happy Labsgiving! Grateful for many things this year including these grad students who organized a surprise sushi lunch 🍣😊
19: Really proud of the whole lab, especially Jeremy and Adam for making it though grant writing and fellowship writing season. As a lab, we've submitted THIRTEEN proposals since the summer. Received two so far, 🤞🏼🤞🏻🤞🏼🤞🏾🤞🏻for more.
09: Big moves in the Campbell Lab!
29: Absolute blast this year celebrating Halloween and carving pumpkins. Winning the "most artistic" category was just the icing on the cake.
25: Picked up our pumpkins today: big plans this year for the Basic Sciences /Human Bio pumpkin carving contest! Thanks to Caleigh for hosting the our first annual pumpking carving. 🎃🎃🎃🎃
25: Next birthday, next cake for Adam 🎂🎂🎂
19: Campbell lab birthday season beings: First up, Christy & Rachel!
15: Over ten years in a box and the next gen Group Meeting Gopher (G.M. Gopher) starts a new life as 'a reminder for who is presenting next week.' Chuffed to carry on this tradition from my time in the Karbstein Lab.
01: Thanks to a generous donation from the Koss Family, we'll be able to pursue an antibody discovery project using the Antibody Technology Shared Resource at Fred Hutch! We're hoping these experiments will help us better understand the mechanism of integrin activation. Proud of this first grant awarded to the lab!
18: Three talks in three days! Different audiences, different lengths, different content.... Probably won't agree to that time line again, but this last one was truly an honor to take part in! Read more about this outstanding partnership here.
17: Campbell Lab's on a ROLL. Even though he was one of the most junior scientists of the 38 participating (rising 2nd year grad student), the extraordinary Adam won first price at the Fred Hutch 40th Anniversary Basic Sciences Retreat Poster Session!!
14: Lab sushi lunch to celebrate the fantastic Jeremy and his well earned T32 Molecular Biophysics Training Program Grant! 🎉
16: Second lab flood (on a much bigger scale than our leaky fridge)!! 🙌 to Singhvi & Bai labs for helping us panic-mop and 🙌🙌🙌to Hutch facilities for cleaning everything up so quickly. Fortunately, we had moved our workstations, etude and scherzo, the previous week.
14: Celebrating our first labbiversary with paddle boarding & pizza.
07: Super excited to host two fantastic seminar speakers this week! Cristina Puchades from UCSF shared her recent innovations, tips, & tricks to solve high resolution structures of membrane proteins, and Daniel Asarnow presented some of the intricate and comprehsive work he has done to characterize a collection of SARS-CoV-2 antibodies structurally and beyond.
First lab pizza party to celebrate two great talks, two submitted fellowships, AND the approved funding for a new carbon evaporator.
06: Two graduate student fellowship proposals submitted and in the books! Couldn't be more proud of Adam & Jeremy for their innovate ideas and getting everything together so quickly after joining to get these shipped.
25: Blast from the past when we did some cleaning up: personalized and monogrammed glass pipettes from when Hal Weintraub himself had his lab in this space over 25 years ago.
10: Both Adam and Jeremy have have joined the lab!
4: First Annual 3rd Floor NE Picnic at Golden Gardens with the Bai & Singhvi Labs was a blast!
First Second Lab Selfie
2: Most entertaining instrument training yet on the BLI. Picked up some fantastic tips for setup and the new software & ended with a group rally to get Adam some last minute extra data for his rotation talk next week.
19: Celebrating first data collection using Leginon on the Talos. Absolutly heroic efforts on the part of Caleigh and the SciComp Team (esp. Ben, Chris, & Russell) to make this happen.
(& also celebrating once again the fact Jeremy makes the best cakes)
12: Will it jinx is if I report that our TC room is really truly completely finished?
11: Grad students are gettin it
24: Melody, Caleigh, and Barry Stoddard talk to GeekWire about the new cryoEM facility at the Hutch!
23: Really grateful to have inherited Lilo and Stitch-- two water jacketed CO2 incubators-- from the Priess lab. They're pulling a lot of weight as we wait for our big new CO2 incubator to be fixed.
19: Celebrating the fact we're all at least partially vaccinated, a full week of gorgeous weather, and the fact Jeremy makes the best cakes.
8: Glacios / K3 update: 2.4Å and aromatic rings!
6: Campbell Lab in Bloom
2: One week into the rotation, and not to brag but check out how good both the grad students are at handling grids, making grids, & staining grids.
29: Welcome to TWO new rotation students: Jeremy Hollis who joins us from the Seattle MCB program and Adam Nguyen from the UW BPSD program. We're so excited to have you both here!
26: Prepping for *two* rotation students to start next week... 😱
Edit: By popular demand, product list can now be found here
25: For a quick introduction for what we do in a cryoEM lab, read what Melody had to say in this interactive partner project between NIGMS and Scholastic: Imaging Lab.
24: Melody talks with Tom Lynch at "Science Says: Tech, terabytes, and transformation." They chat about cryoEM: what it is and what it can do at (and why it could have been advantageous to build the EM Center out at Snoqualmie Pass)
Not up for the full hour of Melody making the extremely talented ASL interpreter's life difficult with phrases like 'high energy electrons,' 'vitreous ice,' and 'electromagnetic interference'? Check out this science snack instead...
19: Just half a day after sign-off, a ~2 hr inagural data collection by Caleigh of an apoferritin sample expressed and purified in the lab, and we have a 2.76Å map using 65 micrographs and ~27k particles.
18: Signed off on the Talos and Glacios electron microscopes today!
4: Welcome to our new "lab member" Scherzo. Ready to process data just as soon as the scopes are complete!
2: Our BLI was installed today-- time to start measuring some binding affinities!
28: No news is good news?
26: Signed off on the Gatan K3 detector for the electron microscope today.
15: It may not be the prettiest first western blot but it's *our* first western blot.
05: New year, new experiments!
22: Warmest* Wishes from the Campbell Lab! 2020 has been a wild time to open a lab, but with support from our neighbors at the Hutch I'm thrilled at what our little lab has set up so far. Excited for what's to come in 2021 with a post-doc joining and grad student rotating in the new year.
17: The glow discharger is up!
15: Our TC setup is up and running and we're growing cells.
11: The FEG has successfully been installed on the Glacios and we have a beam (albeit a very shakey and unstable one).
11: Our Shimadzu HPLC "Chewbacca" was set up today and we're ready* to run some FSEC. Chewbacca joins his new brother-- also called Chewbacca-- as the third HPLC-for-FSEC (to our knowledge) with this name.
03: The Glacios is almost ready for the field emission gun (FEG)! The FEG is the electron source for our microscope and has a maximum accelerating voltage of 200,000 eV. It provides the small coherent beam of electrons that we use to image our samples at high resolutions.
20: The Glacios microscope is plugged in, powered on, and moving!
18: First Campbell Lab protein purified:
13: The lab is looking more like a lab!
12: Glacios progress...
09: The two new electron microscopes-- a Talos L120C G2 and a Glacios Cryo-TEM-- also arrive at the Hutch today!
09: Welcome to Caleigh Azumaya who joins the Hutch as the facility manager for the CryoEM Center
05: Favorite lab accidental order so far: these comically small 2 mL scoops that I had assumed were much larger and ordered for scooping LB...
28: First Campbell Lab pumpkin contest entry:
15: Our FPLC, "BB8" is up and running! Nomenclature (BB8) follows standard chromatography naming conventions established while in the Cheng Lab.
25: Everyone likes a good before/after: Still some work to do, but movement towards finishing the TC room.
24: Big equipment delivery today.
10: Big changes coming to the Hutch EM Center! Today we officially broke ground and rennovations have begun for our new state-of-the-art cryoEM facility!