News


2020 | 2021

September 2021

18: Three talks in three days! Different audiences, different lengths, different content.... Probably won't agree to that time line again, but this last one was truly an honor to take part in! Read more about this outstanding partnership here.

SEP2021

17: Campbell Lab's on a ROLL. Even though he was one of the most junior scientists of the 38 participating (rising 2nd year grad student), the extraordinary Adam won first price at the Fred Hutch 40th Anniversary Basic Sciences Retreat Poster Session!!

40th

Balloons to celebrate Adam's big win!

Luna Yu

14: Lab sushi lunch to celebrate the fantastic Jeremy and his well earned T32 Molecular Biophysics Training Program Grant! 🎉

sushi selfie

You can't tell from the photo but Jeremy and Adam are definitely wearing matching hoodies.


August 2021

16: Second lab flood (on a much bigger scale than our leaky fridge)!!  🙌 to Singhvi & Bai labs for helping us panic-mop and 🙌🙌🙌to Hutch facilities for cleaning everything up so quickly. Fortunately, we had moved our workstations, etude and scherzo, the previous week.

flood1
flood2

July 2021

14: Celebrating our first labbiversary with paddle boarding & pizza.

pb1
bp3
pb2
bp4
pb6
dinner
pizza

11: Finally finished the Glacios time-lapse!

07: Super excited to host two fantastic seminar speakers this week! Cristina Puchades from UCSF shared her recent innovations, tips, & tricks to solve high resolution structures of membrane proteins, and Daniel Asarnow presented some of the intricate and comprehsive work he has done to characterize a collection of SARS-CoV-2 antibodies structurally and beyond.

First lab pizza party to celebrate two great talks, two submitted fellowships, AND the approved funding for a new carbon evaporator.

cristina daniel

06: Two graduate student fellowship proposals submitted and in the books! Couldn't be more proud of Adam & Jeremy for their innovate ideas and getting everything together so quickly after joining to get these shipped.


June 2021

25: Blast from the past when we did some cleaning up: personalized and monogrammed glass pipettes from when Hal Weintraub himself had his lab in this space over 25 years ago.

hal pipettes

10: Both Adam and Jeremy have have joined the lab!

gg jump
Nailed the jump on the first try-- still shocked

4: First Annual 3rd Floor NE Picnic at Golden Gardens with the Bai & Singhvi Labs was a blast!

bai singhvi campbell lab bbq 2021

4: First Second Lab Selfie

selfie

2: Most entertaining instrument training yet on the BLI. Picked up some fantastic tips for setup and the new software & ended with a group rally to get Adam some last minute extra data for his rotation talk next week.

bli
bli2

May 2021

19: Celebrating first data collection using Leginon on the Talos. Absolutly heroic efforts on the part of Caleigh and the SciComp Team (esp. Ben, Chris, & Russell) to make this happen.

leginon talos

(& also celebrating once again the fact Jeremy makes the best cakes)

cake
Three-Layer Fruity Pebble Cake by Jeremy

12: Will it jinx is if I report that our TC room is really truly completely finished?

tissue culture room 1
tissue culture room 2
tissue culture room 3

11: Grad students are gettin it

shaker

April 2021

24: Melody, Caleigh, and Barry Stoddard talk to GeekWire about the new cryoEM facility at the Hutch!

geekwire cryoEM campbell
Melody poses with an empty slide & her least favorite piece of equipment
sunny april jeremy caleigh adam rachel

19: Celebrating the fact we're all at least partially vaccinated, a full week of gorgeous weather, and the fact Jeremy makes the best cakes.

8: Glacios / K3 update: 2.4Å and aromatic rings!

2p4 helix plus

6: Campbell Lab in Bloom

magnolia 2021

2: One week into the rotation, and not to brag but check out how good both the grad students are at handling grids, making grids, & staining grids.

first negative stain
"This is how we used to collect data in the olden days before automation" I explain as I bumble though the low-dose kit on the Talos while we wait for the extremely delayed Leginon server hardware to come in...

March 2021

30: 😎 [Click for PUNS]

integrin smiles
Jeremy's Window Art
smiles smiles
Adam's Biosafety Cabinet Art

29: Welcome to TWO new rotation students: Jeremy Hollis  who joins us from the Seattle MCB program and Adam Nguyen  from the UW BPSD program. We're so excited to have you both here!

 

26: Prepping for *two* rotation students to start next week... 😱

Edit: By popular demand, product list can now be found here

orange group
rainbow

25: For a quick introduction for what we do in a cryoEM lab, read what Melody had to say in this interactive partner project between NIGMS and Scholastic: Imaging Lab.

24: Melody talks with Tom Lynch at "Science Says: Tech, terabytes, and transformation." They chat about cryoEM: what it is and what it can do at (and why it could have been advantageous to build the EM Center out at Snoqualmie Pass)

Science Says: Tech, terabytes, and transformation

Not up for the full hour of Melody making the extremely talented ASL interpreter's life difficult with phrases like 'high energy electrons,' 'vitreous ice,' and 'electromagnetic interference'? Check out this science snack instead...

...the SCIENCE is the snack.

19: Just half a day after sign-off, a ~2 hr inagural data collection by Caleigh of an apoferritin sample expressed and purified in the lab, and we have a 2.76Å map using 65 micrographs and ~27k particles.

apoF 2p76

18: Signed off on the Talos and Glacios electron microscopes today!

4: Welcome to our new "lab member" Scherzo. Ready to process data just as soon as the scopes are complete!

going
how it's going
huitzi
(how it started)

2: Our BLI was installed today-- time to start measuring some binding affinities!

octet

February 2021

28: No news is good news?


January 2021

26: Signed off on the Gatan K3 detector for the electron microscope today.

15: It may not be the prettiest first western blot but it's *our* first western blot.

dino western blot

05: New year, new experiments!

first experiment

When growing mammlian cells keeping an ideal environment is extremely important for them to survive. We keep these cells on a moving shaker to help them stay aerated, and the shaker is inside of an incubator that's like south Florida in July: 37 ˚C and >80% humdity.


December 2020

22: Warmest* Wishes from the Campbell Lab! 2020 has been a wild time to open a lab, but with support from our neighbors at the Hutch I'm thrilled at what our little lab has set up so far. Excited for what's to come in 2021 with a post-doc joining and grad student rotating in the new year.

Warmest wishes from the campbell lab 2020

These little snowpeople are at nearly ≥98% viability & almost ready for transfection

17: The glow discharger is up!

glow discharger

glow dischargers gonna glow: A current of of 15 mA is passed though the chamber at low pressure which transfers energy to the electrons in gas molecules in the air. There is enough energy to generate the purple luminosity and ions are deposited on to anything within the chamber (in our case a EM grid). This makes the surface of the grid hydrophilic (negatively charged) and causes aqueous solutions to spread across the surface. This spreading helps us attain well dispersed particles for negative-stain imaging and thin even ice for cryo imaging.

15: Our TC setup is up and running and we're growing cells.

expi293 december 2020

Our Expi293 cells alive (mostly) & dividing.

11: The FEG has successfully been installed on the Glacios and we have a beam (albeit a very shakey and unstable one).

feg installed

& we didn't drop the FEG!

11: Our Shimadzu HPLC "Chewbacca" was set up today and we're ready* to run some FSEC. Chewbacca joins his new brother-- also called Chewbacca-- as the third HPLC-for-FSEC (to our knowledge) with this name.

han solos friend

*still need GFP-tagged protein

03: The Glacios is almost ready for the field emission gun (FEG)! The FEG is the electron source for our microscope and has a maximum accelerating voltage of 200,000 eV. It provides the small coherent beam of electrons that we use to image our samples at high resolutions.

feg prep

But what are the cupcake pans for?


November 2020

20: The Glacios microscope is plugged in, powered on, and moving!

The stage is moving, not the whole microscope. That would be bad. Video by Caleigh Azumaya

18: First Campbell Lab protein purified:

its just a protease to cleave tags

Maybe just a small victory for now, but this it felt like a huge relief because it means that we've sucessfully set up cell growth & protein expression and that the cells, media, buffers, incubators, FPLC, columns, and gels are all working as expected.

first gel
Images (& protein purification) by Rachel Werther

13: The lab is looking more like a lab!

how its going

This, of course, is Rachel's bench. Mine is not as tidy...

12: Glacios progress...

do we cut the green or red wires

A+ cable management

09: The two new electron microscopes-- a Talos L120C G2 and a Glacios Cryo-TEM-- also arrive at the Hutch today!

09: Welcome to Caleigh Azumaya who joins the Hutch as the facility manager for the CryoEM Center

melody the glacios and caleigh delivery

Left to Right: Glacios Box #1, Melody, Glacios Box #2, Caleigh, Glacios Box #3. The box between Melody and Caleigh weighs 925 kg (2040 lbs/ 1 rhinoceros) and pictured are only 3 of the 22 boxes delivered for the two microscopes.

Photo by Rachel Werther

05: Favorite lab accidental order so far: these comically small 2 mL scoops that I had assumed were much larger and ordered for scooping LB...

2mL scoops

Does this really count as news?


October 2020

28: First Campbell Lab pumpkin contest entry:

zoom pumpkin

15: Our FPLC, "BB8" is up and running! Nomenclature (BB8) follows standard chromatography naming conventions established while in the Cheng Lab.

BB8 Akta

Now just waiting for the column and injection kit to come in....

05: Welcome to our new technician, Rachel Werther, who joins us from Barry Stoddard's lab.


September 2020

25: Everyone likes a good before/after: Still some work to do, but movement towards finishing the TC room.

pre 2
before 2
after2

#glowup

24: Big equipment delivery today.

akta pure

2: Melody speaks with Tom Lynch at the Fred Hutch Virtual Town Hall about plans for the new CryoEM Center @ Fred hutch. Read the Full transcript here.


August 2020

10: Big changes coming to the Hutch EM Center! Today we officially broke ground and rennovations have begun for our new state-of-the-art cryoEM facility!

construction 01
construction 02

July 2020

01: Campbell Lab opens & we're hiring at all levels!

empty campbell lab photo july 2020

Ten empty lab benches waiting to be filled!