18: Three talks in three days! Different audiences, different lengths, different content.... Probably won't agree to that time line again, but this last one was truly an honor to take part in! Read more about this outstanding partnership here.
17: Campbell Lab's on a ROLL. Even though he was one of the most junior scientists of the 38 participating (rising 2nd year grad student), the extraordinary Adam won first price at the Fred Hutch 40th Anniversary Basic Sciences Retreat Poster Session!!
14: Lab sushi lunch to celebrate the fantastic Jeremy and his well earned T32 Molecular Biophysics Training Program Grant! 🎉
16: Second lab flood (on a much bigger scale than our leaky fridge)!! 🙌 to Singhvi & Bai labs for helping us panic-mop and 🙌🙌🙌to Hutch facilities for cleaning everything up so quickly. Fortunately, we had moved our workstations, etude and scherzo, the previous week.
14: Celebrating our first labbiversary with paddle boarding & pizza.
07: Super excited to host two fantastic seminar speakers this week! Cristina Puchades from UCSF shared her recent innovations, tips, & tricks to solve high resolution structures of membrane proteins, and Daniel Asarnow presented some of the intricate and comprehsive work he has done to characterize a collection of SARS-CoV-2 antibodies structurally and beyond.
First lab pizza party to celebrate two great talks, two submitted fellowships, AND the approved funding for a new carbon evaporator.
06: Two graduate student fellowship proposals submitted and in the books! Couldn't be more proud of Adam & Jeremy for their innovate ideas and getting everything together so quickly after joining to get these shipped.
25: Blast from the past when we did some cleaning up: personalized and monogrammed glass pipettes from when Hal Weintraub himself had his lab in this space over 25 years ago.
10: Both Adam and Jeremy have have joined the lab!
4: First Annual 3rd Floor NE Picnic at Golden Gardens with the Bai & Singhvi Labs was a blast!
First Second Lab Selfie
2: Most entertaining instrument training yet on the BLI. Picked up some fantastic tips for setup and the new software & ended with a group rally to get Adam some last minute extra data for his rotation talk next week.
19: Celebrating first data collection using Leginon on the Talos. Absolutly heroic efforts on the part of Caleigh and the SciComp Team (esp. Ben, Chris, & Russell) to make this happen.
(& also celebrating once again the fact Jeremy makes the best cakes)
12: Will it jinx is if I report that our TC room is really truly completely finished?
11: Grad students are gettin it
19: Celebrating the fact we're all at least partially vaccinated, a full week of gorgeous weather, and the fact Jeremy makes the best cakes.
8: Glacios / K3 update: 2.4Å and aromatic rings!
6: Campbell Lab in Bloom
2: One week into the rotation, and not to brag but check out how good both the grad students are at handling grids, making grids, & staining grids.
29: Welcome to TWO new rotation students: Jeremy Hollis who joins us from the Seattle MCB program and Adam Nguyen from the UW BPSD program. We're so excited to have you both here!
26: Prepping for *two* rotation students to start next week... 😱
Edit: By popular demand, product list can now be found here
25: For a quick introduction for what we do in a cryoEM lab, read what Melody had to say in this interactive partner project between NIGMS and Scholastic: Imaging Lab.
24: Melody talks with Tom Lynch at "Science Says: Tech, terabytes, and transformation." They chat about cryoEM: what it is and what it can do at (and why it could have been advantageous to build the EM Center out at Snoqualmie Pass)
Not up for the full hour of Melody making the extremely talented ASL interpreter's life difficult with phrases like 'high energy electrons,' 'vitreous ice,' and 'electromagnetic interference'? Check out this science snack instead...
19: Just half a day after sign-off, a ~2 hr inagural data collection by Caleigh of an apoferritin sample expressed and purified in the lab, and we have a 2.76Å map using 65 micrographs and ~27k particles.
18: Signed off on the Talos and Glacios electron microscopes today!
4: Welcome to our new "lab member" Scherzo. Ready to process data just as soon as the scopes are complete!
2: Our BLI was installed today-- time to start measuring some binding affinities!
28: No news is good news?
26: Signed off on the Gatan K3 detector for the electron microscope today.
15: It may not be the prettiest first western blot but it's *our* first western blot.
05: New year, new experiments!
22: Warmest* Wishes from the Campbell Lab! 2020 has been a wild time to open a lab, but with support from our neighbors at the Hutch I'm thrilled at what our little lab has set up so far. Excited for what's to come in 2021 with a post-doc joining and grad student rotating in the new year.
17: The glow discharger is up!
15: Our TC setup is up and running and we're growing cells.
11: The FEG has successfully been installed on the Glacios and we have a beam (albeit a very shakey and unstable one).
11: Our Shimadzu HPLC "Chewbacca" was set up today and we're ready* to run some FSEC. Chewbacca joins his new brother-- also called Chewbacca-- as the third HPLC-for-FSEC (to our knowledge) with this name.
03: The Glacios is almost ready for the field emission gun (FEG)! The FEG is the electron source for our microscope and has a maximum accelerating voltage of 200,000 eV. It provides the small coherent beam of electrons that we use to image our samples at high resolutions.
20: The Glacios microscope is plugged in, powered on, and moving!
18: First Campbell Lab protein purified:
13: The lab is looking more like a lab!
12: Glacios progress...
09: The two new electron microscopes-- a Talos L120C G2 and a Glacios Cryo-TEM-- also arrive at the Hutch today!
09: Welcome to Caleigh Azumaya who joins the Hutch as the facility manager for the CryoEM Center
05: Favorite lab accidental order so far: these comically small 2 mL scoops that I had assumed were much larger and ordered for scooping LB...
28: First Campbell Lab pumpkin contest entry:
15: Our FPLC, "BB8" is up and running! Nomenclature (BB8) follows standard chromatography naming conventions established while in the Cheng Lab.
25: Everyone likes a good before/after: Still some work to do, but movement towards finishing the TC room.
24: Big equipment delivery today.
10: Big changes coming to the Hutch EM Center! Today we officially broke ground and rennovations have begun for our new state-of-the-art cryoEM facility!