Structures & Datasets
See our most recent structures here.
CryoEM Structures
Integrin α5β1 in complex with Fab BIIG2 Fab
Map: EMD-44386
Model: PDB: 9B9J (fab xtal structure: PDB: 8R38)
Datasets: Coming Soon
Nguyen A, Heim JB, Cordara G, Chan MC, Johannesen H, Charlesworth C, Li M, Azumaya CM, Madden B, Krengel U, Meves A, Campbell MG "Structural and functional characterization of integrin α5-targeting antibodies for anti-angiogenic therapy" 2025 Jan 08 https://www.biorxiv.org/content/10.1101/2025.01.08.631572v1
Integrin α5β1 in complex with MINT 1526A Fab
Map: EMD-44387
Model: PDB: 9B9K
Datasets: Coming Soon
Nguyen A, Heim JB, Cordara G, Chan MC, Johannesen H, Charlesworth C, Li M, Azumaya CM, Madden B, Krengel U, Meves A, Campbell MG "Structural and functional characterization of integrin α5-targeting antibodies for anti-angiogenic therapy" 2025 Jan 08 https://www.biorxiv.org/content/10.1101/2025.01.08.631572v1
Integrin α5β1 in complex with NeoNectin
Map: EMD-45655
Model: PDB: 9CKV
Datasets: Coming Soon
Wang X, Guillem-Marti J, Kumar S, Lee DS, Cabrerizo-Aguado D, Werther R, Estrado Alamo KA, Zhao YT, Nguyen A, Kopyeva I, Huang B, Li J, Hao Y, Li X, Brizuela-Velasco A, Murray AN, Gerben S, Roy A, DeForest CA, Springer TA, Ruohola-Baker H, Cooper JA, Campbell MG, Manero JM, Ginebra MP, Baker D. "De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine" bioRxiv. 2024 June 25. doi: https://doi.org/10.1101/2024.06.21.600123
Integrin α5β1 in complex with NeoNectin candidate 5.3 (Closed)
Map: EMDB-46902
Model: PDB 9DIA
Datasets: Coming Soon
Wang X, Guillem-Marti J, Kumar S, Lee DS, Cabrerizo-Aguado D, Werther R, Estrado Alamo KA, Zhao YT, Nguyen A, Kopyeva I, Huang B, Li J, Hao Y, Li X, Brizuela-Velasco A, Murray AN, Gerben S, Roy A, DeForest CA, Springer TA, Ruohola-Baker H, Cooper JA, Campbell MG, Manero JM, Ginebra MP, Baker D. "De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine" bioRxiv. 2024 June 25. doi: https://doi.org/10.1101/2024.06.21.600123
Integrin α5β1 in complex with NeoNectin candidate 5.3 (Open)
Map: EMDB-47968
Model: PDB 9EF2
Datasets: Coming Soon
Wang X, Guillem-Marti J, Kumar S, Lee DS, Cabrerizo-Aguado D, Werther R, Estrado Alamo KA, Zhao YT, Nguyen A, Kopyeva I, Huang B, Li J, Hao Y, Li X, Brizuela-Velasco A, Murray AN, Gerben S, Roy A, DeForest CA, Springer TA, Ruohola-Baker H, Cooper JA, Campbell MG, Manero JM, Ginebra MP, Baker D. "De Novo Design of Integrin α5β1 Modulating Proteins for Regenerative Medicine" bioRxiv. 2024 June 25. doi: https://doi.org/10.1101/2024.06.21.600123
Integrin αEβ7 in complex with E-cadherin
Map: Coming Soon
Model: PDB: Coming Soon
Datasets: Coming Soon
Hollis JA, Chan MC, Malik HS, Campbell MG. "Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins" bioRxiv. 2023 November 05. doi: https://doi.org/10.1101/2023.11.05.565221
Integrin αEβ7 alone (open)
Map: Coming Soon
Model: PDB: Coming Soon
Datasets: Coming Soon
Hollis JA, Chan MC, Malik HS, Campbell MG. "Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins" bioRxiv. 2023 November 05. doi: https://doi.org/10.1101/2023.11.05.565221
Integrin αEβ7 alone (closed)
Map: Coming Soon
Model: PDB: Coming Soon
Datasets: Coming Soon
Hollis JA, Chan MC, Malik HS, Campbell MG. "Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins" bioRxiv. 2023 November 05. doi: https://doi.org/10.1101/2023.11.05.565221
Integrin α4β7 in complex with MadCAM-1
Map: Coming Soon
Model: PDB: Coming Soon
Datasets: Coming Soon
Hollis JA, Chan MC, Malik HS, Campbell MG. "Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins" bioRxiv. 2023 November 05. doi: https://doi.org/10.1101/2023.11.05.565221
Integrin α4β7 alone
Map: Coming Soon
Model: PDB: Coming Soon
Datasets: Coming Soon
Hollis JA, Chan MC, Malik HS, Campbell MG. "Evolutionary origin and structural ligand mimicry by the inserted domain of alpha-integrin proteins" bioRxiv. 2023 November 05. doi: https://doi.org/10.1101/2023.11.05.565221
Integrin alpha-v beta-6 in complex with minibinder B6_BP_dslf
Map: EMD-41154
Model: PDB: 8tcg
Datasets: EMPIAR-12689
Roy A*, Shi L*, Chang A*, Dong X, Fernandez A, Kraft JC, Li J, Le VQ, Winegar RV, Cherf GM, Slocum D, Poulson PD, Casper GE, Vallecillo-Zuniga ML, Valdoz JC, Miranda MC, Bai H, Kipnis Y, Olshefsky A, Priya T, Carter L, Ravichandran T, Chow C, Johnson MR, Chang A, Overed-Sayer C, Finch DK, Lowe D, Bera AK, Matute-Bello G, Birkland TP, DiMaio F, Raghu G, Cochran JR, Stewart L, Campbell MG#, Van Ry PM#, Springer TA#, Baker D#. "De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8" Nat Commun. 2023 Sep 13;14(1):5660. doi: 10.1038/s41467-023-41272-z. PMID: 37704610
Integrin alpha-v beta-8 in complex with minibinder B8_BP_dslf
Map: EMD-41153
Model: PDB: 8tcf
Datasets: EMPIAR-12690
Roy A*, Shi L*, Chang A*, Dong X, Fernandez A, Kraft JC, Li J, Le VQ, Winegar RV, Cherf GM, Slocum D, Poulson PD, Casper GE, Vallecillo-Zuniga ML, Valdoz JC, Miranda MC, Bai H, Kipnis Y, Olshefsky A, Priya T, Carter L, Ravichandran T, Chow C, Johnson MR, Chang A, Overed-Sayer C, Finch DK, Lowe D, Bera AK, Matute-Bello G, Birkland TP, DiMaio F, Raghu G, Cochran JR, Stewart L, Campbell MG#, Van Ry PM#, Springer TA#, Baker D#. "De novo design of highly selective miniprotein inhibitors of integrins αvβ6 and αvβ8" Nat Commun. 2023 Sep 13;14(1):5660. doi: 10.1038/s41467-023-41272-z. PMID: 37704610
Conformational snapshots of αvβ8 integrin bound to L-TGFβ
Maps: EMD-20794, EMD-20797 to EMD-20802 & EMD-21125
Model: PDB: 6uja
Datasets: EMPIAR-10343 & EMPIAR-10344
Campbell MG, Cormier A, Ito S, Seed RI, Bondesson AJ, Lou J, Marks JD, Baron JL, Cheng Y, Nishimura SL. "Cryo-EM reveals a mechanism of integrin-mediated TGF-β activation within the latent TGF-β complex." Cell. 2020 Feb 6;180(3):490-501.e16.
αvβ8 Integrin bound to fabs C6D4 and 11D12v2
Map: EMD-20795
Model: PDB: 6ujab
Dataset: EMPIAR-10345
Campbell MG, Cormier A, Ito S, Seed RI, Bondesson AJ, Lou J, Marks JD, Baron JL, Cheng Y, Nishimura SL. "Cryo-EM reveals a mechanism of integrin-mediated TGF-β activation within the latent TGF-β complex." Cell. 2020 Feb 6;180(3):490-501.e16.
αvβ8 integrin bound to fabs RGD3 and 11D12v2
Map: EMD-20796
Model: PDB: 6ujc
Campbell MG, Cormier A, Ito S, Seed RI, Bondesson AJ, Lou J, Marks JD, Baron JL, Cheng Y, Nishimura SL. "Cryo-EM reveals a mechanism of integrin-mediated TGF-β activation within the latent TGF-β complex." Cell. 2020 Feb 6;180(3):490-501.e16.
Conformational Snapshots of αvβ8 Integrin in complex with the Fabs 68 and 8B8
Map: EMD-7939
Model: PDB: 6djp
Cormier A, Campbell MG, Ito S, Wu S, Lou J, Marks J, Baron J, Nishimura SL, Cheng Y. "Cryo-EM structure of the αvβ8 integrin reveals a mechanism for stabilizing integrin extension." Nat Struct Mol Biol. 2018; 25(8):698–704.
Human TRPM4 ion channel in lipid nanodiscs
Maps: EMD-7132 & EMD-7133
Models: PDB: 6bqr & 6bqv
Datasets: EMPIAR-10126 & EMPIAR-10127
Autzen HE, Myasnikov AG, Campbell MG, Asarnow D, Julius D, Cheng Y. "Structure of the human TRPM4 ion channel in a lipid nanodisc." Science. 2018; 359 (6372), 228-232.
Human adenovirus 26
Maps: EMD-8471
Model: PDB: 5tx1
Yu X, Veesler D, Campbell MG, Barry ME, Asturias FJ, Barry MA and Reddy VS. "Cryo-EM structure of human adenovirus D26 reveals the conservation of structural organization among human adenoviruses." Science Advances. 2017; 3(5):e1602670.
20S proteasome
Map: EMD-6287
Model: PDB: 6bdf
Dataset: EMPIAR-10025
Campbell MG, Veesler D, Cheng A, Potter CS, Carragher B. "2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy." eLife. 2015; 4:e06380.
Nudaurelia capensis omega virus (NwV)
Map: EMD-2791
Campbell MG, Kearney BM, Cheng A, Potter CS, Johnson JE, Carragher B, Veesler D. "Near-atomic resolution reconstructions using a mid-range electron microscope operated at 200kV." J Struct Biol. 2014; 188(2):183-187.
20S proteasome
Map: EMD-2792
Campbell MG, Kearney BM, Cheng A, Potter CS, Johnson JE, Carragher B, Veesler D. "Near-atomic resolution reconstructions using a mid-range electron microscope operated at 200kV." J Struct Biol. 2014; 188(2):183-187.
Sulfolobus turreted icosahedral virus (STIV)
Map: EMD-5754
Veesler D, Campbell MG, Cheng A, Fu C-Y, Murez Z, Johnson JE, Potter CS, Carragher B. "Maximizing the potential of electron cryomicroscopy data collected using direct detectors." J Struct Biol. 2013; 184(2):193–202.
Rotavirus Double Layer Particle without motion correction
Maps: EMD-5485 (Full); EMD-5487 (Spike)
Campbell MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS, Carragher B, Grigorieff N. "Movies of ice-embedded particles enhance resolution in electron cryo-microscopy." Structure. 2012; 20(11):1823–1828.
Rotavirus Double Layer Particle with motion correction
Maps: EMD-5486 (Full); EMD-5488 (Spike)
Campbell MG, Cheng A, Brilot AF, Moeller A, Lyumkis D, Veesler D, Pan J, Harrison SC, Potter CS, Carragher B, Grigorieff N. "Movies of ice-embedded particles enhance resolution in electron cryo-microscopy." Structure. 2012; 20(11):1823–1828.
Negative Stain EM Structures
ZMET2 with H3Kc9me3 dinucleosome and DNA linker
Map: EMD-9127
Stoddard CI, Feng S, Campbell MG, Liu W, Wang H, Zhong X, Bernatavichute Y, Cheng Y, Jacobsen SE, Narlikar GJ. "A plant maintenance DNA methyltransferase is activated by the combinatorial action of chromatin architecture, histone methylation and DNA methylation." Mol Cell. 2019; 73(1):73-83.e6.
Conformational Snapshots of Inducible Nitric Oxide Synthase (iNOS)
Maps: EMD-2718 to EMD-2749
Campbell MG, Smith BC, Potter CS, Carragher B, Marletta MA. "Molecular architecture of mammalian nitric oxide synthases." Proc Natl Acad Sci U S A. 2014;111(35):E3614–23
Conformational Snapshots of apo Soluble Gunalyate Cyclase (sGC)
Maps: EMD-5861 to EMD-5878
Campbell MG, Underbakke ES, Potter CS, Carragher B, Marletta MA. "Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase." Proc Natl Acad Sci U S A. 2014; 111(8):2960–2965.
Conformational Snapshots of Soluble Gunalyate Cyclase (sGC) bound to nitric oxide
Maps: EMD-5879 to EMD-5880
Campbell MG, Underbakke ES, Potter CS, Carragher B, Marletta MA. "Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase." Proc Natl Acad Sci U S A. 2014; 111(8):2960–2965.
Conformational Snapshots of Soluble Gunalyate Cyclase (sGC) bound to GPCPP
Maps: EMD-5881 to EMD-5884
Campbell MG, Underbakke ES, Potter CS, Carragher B, Marletta MA. "Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase." Proc Natl Acad Sci U S A. 2014; 111(8):2960–2965.
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